SDS-Page
RPS12 recombinant protein
RPS12, 1-132aa, Human, His tag, E Coli
Gene Names
RPS12; S12
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
RPS12; 1-132aa; Human; His tag; E Coli; 40S ribosomal protein S12; S12; RPS12 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEEGIA AGGVMDVNTA LQEVLKTALI HDGLARGIRE AAKALDKRQA HLCVLASNCD EPMYVKLVEA LCAEHQINLI KVDDNKKLGE WVGLCKIDRE GKPRKVVGCS CVVVKDYGKE SQAKDVIEEY FKCKK
Sequence Length
132
Applicable Applications for RPS12 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for RPS12 recombinant protein
RPS12 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPS12 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for RPS12 recombinant protein
References
Yoshihama M, uechi T, et al. (2002). Genome Res.12 (3):379-90.; Yu Y, Ji H, Doudna JA, et al. (2005). Protein Sci. 14 (6):1438-46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16.9kDa (155aa), confirmed by MALDI-TOF
NCBI Official Full Name
40S ribosomal protein S12
NCBI Official Synonym Full Names
ribosomal protein S12
NCBI Official Symbol
RPS12
NCBI Official Synonym Symbols
S12
NCBI Protein Information
40S ribosomal protein S12
UniProt Protein Name
40S ribosomal protein S12
Protein Family
UniProt Gene Name
RPS12
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Research Articles on RPS12
Similar Products
Product Notes
The RPS12 rps12 (Catalog #AAA205281) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's RPS12 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RPS12 rps12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAEEGIA AGGVMDVNTA LQEVLKTALI HDGLARGIRE AAKALDKRQA HLCVLASNCD EPMYVKLVEA LCAEHQINLI KVDDNKKLGE WVGLCKIDRE GKPRKVVGCS CVVVKDYGKE SQAKDVIEEY FKCKK. It is sometimes possible for the material contained within the vial of "RPS12, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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