BuB3 recombinant protein

BuB3, 1-328aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205204

• Gene Names: BUB3; BUB3L; hBUB3
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: BuB3; 1-328aa; Human; His tag; E Coli (Denatured); Mitotic checkpoint protein BuB3; BuB3L; hBuB3; BuB3 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T
• Sequence Length: 328

• Applicable Applications for BuB3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BuB3 recombinant protein

Mitotic checkpoint protein BuB3, also known as BuB3, is a conserved component of the mitotic spindle assembly complex (MCC). The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BuB3 protein. BuB3 is essential for the kinetochore localization of BuB1 and BuBR1. BuB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. In addition, BuB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. Recombinant human BuB3 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for BuB3 recombinant protein

Cell Cycle

References

Martinez Exposito M J., et al. (1999) Proc Natl Acad Sci uSA. 96:8493-8498.; Warren C D., et al. (2002) Mol Biol Cell. 13:3029-3041.

NCBI and Uniprot Product Information

• NCBI GI #: 4757880
• NCBI GeneID: 9184
• NCBI Accession #: NP_004716
• NCBI GenBank Nucleotide #: NM_004725.3
• UniProt Accession #: O43684; O43685; A6NJ42; B2R6E7; D3DRE9
• Molecular Weight: 39.5 kDa (351aa)
• NCBI Official Full Name: mitotic checkpoint protein BUB3 isoform a
• NCBI Official Synonym Full Names: BUB3, mitotic checkpoint protein
• NCBI Official Symbol: BUB3
• NCBI Official Synonym Symbols: BUB3L; hBUB3
• NCBI Protein Information: mitotic checkpoint protein BUB3
• UniProt Protein Name: Mitotic checkpoint protein BUB3
• Protein Family: BUB3-interacting and GLEBS motif-containing protein
• UniProt Gene Name: BUB3

NCBI Description

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.