CCM2 recombinant protein

CCM2, 66-353aa, Human, His tag, E Coli

Cat. Num. AAA205127

• Gene Names: CCM2; OSM; C7orf22; PP10187
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CCM2; 66-353aa; Human; His tag; E Coli; Malcavernin isoform 4; CCM2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEVKYLGQ LTSIPGYLNP SSRTEILHFI DNAKRAHQLP GHLTQEHDAV LSLSAYNVKL AWRDGEDIIL RVPIHDIAAV SYVRDDAAHL VVLKTDDSST KVDIKETYEV EASTFCFPES VDVGGASPHS KTISESELSA SATELLQDYM LTLRTKLSSQ EIQQFAALLH EYRNGASIHE FCINLRQLYG DSRKFLLLGL RPFIPEKDSQ HFENFLETIG VKDGRGIITD SFGRHRRALS TTSSSTTNGN RATGSSDDRS APSEGDEWDR MISDISSDIE ALGCSMDQDS A
• Sequence Length: 353

• Applicable Applications for CCM2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CCM2 recombinant protein

CCM2 provides instructions for making a protein called malcavernin, which strengthens the interactions between cells that form blood vessels and limits leakage from the vessels. It interacts with a number of other proteins to form a complex that is found in the junctions that connect neighboring cells. It helps turn off (suppress) a signaling molecule known as RhoA-GTPase. Recombinant human CCM2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for CCM2 recombinant protein

Signal Transduction

References

Dupre N. et al. (2003) Can J Neurol Sci. 30:122-128; Liquori CL. et al. (2007) Am J Hum Genet. 80:69-75.;

NCBI and Uniprot Product Information

• NCBI GI #: 269308190
• NCBI GeneID: 83605
• NCBI Accession #: NP_001161407
• NCBI GenBank Nucleotide #: NM_001167935.1
• UniProt Accession #: Q9BSQ5; Q71RE5; Q8TAT4; A4D2L4; B3KUV0; D3DVL4; E9PDJ3; F5H0E1; F5H551
• Molecular Weight: 34.3kDa (311aa) confirmed by MALDI-TOF
• NCBI Official Full Name: cerebral cavernous malformations 2 protein isoform 4
• NCBI Official Synonym Full Names: CCM2 scaffolding protein
• NCBI Official Symbol: CCM2
• NCBI Official Synonym Symbols: OSM; C7orf22; PP10187
• NCBI Protein Information: cerebral cavernous malformations 2 protein
• UniProt Protein Name: Cerebral cavernous malformations 2 protein
• Protein Family: Cerebral cavernous malformations protein
• UniProt Gene Name: CCM2
• UniProt Synonym Gene Names: C7orf22

NCBI Description

This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]

Uniprot Description

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions (). May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock ().