RAB10 recombinant protein

RAB10, 1-200aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205034

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RAB10; 1-200aa; Human; His tag; E Coli (Denatured); Ras-related GTP-binding protein RAB10; RAB10 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.4M urea
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC
• Sequence Length: 200

• Applicable Applications for RAB10 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAB10 recombinant protein

RAB10 is a member of the Ras superfamily of small GTP-binding proteins. This protein is a key regulator of endocytic recycling, especially in the basolateral recycling pathways of polarized epithelial cells. It is required for the recruitment of CNT-1 to endosomal membranes in the intestinal epithelium. Recombinant human RAB10 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for RAB10 recombinant protein

Signal Transduction

References

Chen Y. et al. (2012) J Cell Biol. 198:545-560.; Sano H. et al. (2011) J Biol Chem. 286:16541-16545.

NCBI and Uniprot Product Information

• NCBI GI #: 256222019
• NCBI GeneID: 10890
• NCBI Accession #: NP_057215
• NCBI GenBank Nucleotide #: NM_016131.4
• UniProt Accession #: P61026; O88386; Q6IA52; Q9D7X6; Q9H0T3; D6W538
• Molecular Weight: 24.7 kDa (220aa)
• NCBI Official Full Name: ras-related protein Rab-10
• NCBI Official Synonym Full Names: RAB10, member RAS oncogene family
• NCBI Official Symbol: RAB10
• NCBI Protein Information: ras-related protein Rab-10
• UniProt Protein Name: Ras-related protein Rab-10
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB10

NCBI Description

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).[supplied by OMIM, Mar 2009]

Uniprot Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.