GMPS recombinant protein

GMPS, 1-693aa, Human, His tag, E Coli

Cat. Num. AAA204738

• Gene Names: GMPS; GATD7
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GMPS; 1-693aa; Human; His tag; E Coli; GMP synthase [glutamine-hydrolyzing]; Glutamine amidotransferase; guanine monophosphate synthetase; GMPS recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMALCNG DSKLENAGGD LKDGHHHYEG AVVILDAGAQ YGKVIDRRVR ELFVQSEIFP LETPAFAIKE QGFRAIIISG GPNSVYAEDA PWFDPAIFTI GKPVLGICYG MQMMNKVFGG TVHKKSVRED GVFNISVDNT CSLFRGLQKE EVVLLTHGDS VDKVADGFKV VARSGNIVAG IANESKKLYG AQFHPEVGLT ENGKVILKNF LYDIAGCSGT FTVQNRELEC IREIKERVGT SKVLVLLSGG VDSTVCTALL NRALNQEQVI AVHIDNGFMR KRESQSVEEA LKKLGIQVKV INAAHSFYNG TTTLPISDED RTPRKRISKT LNMTTSPEEK RKIIGDTFVK IANEVIGEMN LKPEEVFLAQ GTLRPDLIES ASLVASGKAE LIKTHHNDTE LIRKLREEGK VIEPLKDFHK DEVRILGREL GLPEELVSRH PFPGPGLAIR VICAEEPYIC KDFPETNNIL KIVADFSASV KKPHTLLQRV KACTTEEDQE KLMQITSLHS LNAFLLPIKT VGVQGDCRSY SYVCGISSKD EPDWESLIFL ARLIPRMCHN VNRVVYIFGP PVKEPPTDVT PTFLTTGVLS TLRQADFEAH NILRESGYAG KISQMPVILT PLHFDRDPLQ KQPSCQRSVV IRTFITSDFM TGIPATPGNE IPVEVVLKMV TEIKKIPGIS RIMYDLTSKP PGTTEWE
• Sequence Length: 693

• Applicable Applications for GMPS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GMPS recombinant protein

GMP synthase, also known as GMPS, is involved in purine biosynthesis. Existing as a homodimer, GMPS catalyzes the last step in the GMP synthesis pathway, namely the ATP-dependent amination of XMP to GMP. GMPS contains one GMP-binding domain and one glutamine amidotransferase type-1 domain through which it conveys its catalytic activity. Chromosomal translocations involving the gene encoding GMPS are associated with acute myeloid leukemias, suggesting a possible role for GMPS in carcinogenesis. Recombinant human GMPS protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GMPS recombinant protein

Metabolism

References

Hirst M., et al. (1994) J Biol Chem. 269:23830-23837.; Nakamura J., et al. (1995) J Biol Chem. 270:23450-23455.

NCBI and Uniprot Product Information

• NCBI GI #: 4504035
• NCBI GeneID: 8833
• NCBI Accession #: NP_003866
• NCBI GenBank Nucleotide #: NM_003875.2
• UniProt Accession #: P49915; A8K639; B4DXV7; F8W720
• Molecular Weight: 79.2 kDa (717aa)
• NCBI Official Full Name: GMP synthase
• NCBI Official Synonym Full Names: guanine monophosphate synthase
• NCBI Official Symbol: GMPS
• NCBI Official Synonym Symbols: GATD7
• NCBI Protein Information: GMP synthase [glutamine-hydrolyzing]
• UniProt Protein Name: GMP synthase [glutamine-hydrolyzing]
• UniProt Gene Name: GMPS

NCBI Description

In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.