uBL4A recombinant protein

uBL4A, 1-157aa, Human, His tag, E Coli

Cat. Num. AAA204732

• Gene Names: UBL4A; GDX; G6PD; GET5; MDY2; UBL4; TMA24; DX254E; DXS254E
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: uBL4A; 1-157aa; Human; His tag; E Coli; ubiquitin-like protein 4A; G6PD; GDX; GET5; MDY2; TMA24; uBL4; uBL4A recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MQLTVKALQG RECSLQVPED ELVSTLKQLV SEKLNVPVRQ QRLLFKGKAL ADGKRLSDYS IGPNSKLNLV VKPLEKVLLE EGEAQRLADS PPPQVWQLIS KVLARHFSAA DASRVLEQLQ RDYERSLSRL TLDDIERLAS RFLHPEVTET MEKGFSKLEH HHHHH
• Sequence Length: 157

• Applicable Applications for uBL4A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uBL4A recombinant protein

uBL4A, also known as GDX, contains 1 ubiquitin-like domain. Post-translational modification by ubiquitin and ubiquitin-related proteins plays critical roles in protein degradation and in regulation of essential cellular processes. In mammals, transcription grinds to a halt during late spermiogenesis due to compaction of the spermatid genome, which creates a special need for robust post-transcriptional regulation. uBL4A plays any role in targeting cellular proteins for degradation. Recombinant human uBL4A protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for uBL4A recombinant protein

Ubiquitination

References

Yang F, et al. (2007) Gene Expr Patterns. 7 (1-2):131-6. ; Mariappan M., et al. (2010) Nature 466:1120-1124

NCBI and Uniprot Product Information

• NCBI GI #: 7657667
• NCBI GeneID: 8266
• NCBI Accession #: NP_055050
• NCBI GenBank Nucleotide #: NM_014235.4
• UniProt Accession #: P11441; Q5HY80
• Molecular Weight: 18.8kDa (165aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ubiquitin-like protein 4A
• NCBI Official Synonym Full Names: ubiquitin like 4A
• NCBI Official Symbol: UBL4A
• NCBI Official Synonym Symbols: GDX; G6PD; GET5; MDY2; UBL4; TMA24; DX254E; DXS254E
• NCBI Protein Information: ubiquitin-like protein 4A
• UniProt Protein Name: Ubiquitin-like protein 4A
• Protein Family: Ubiquitin-like protein
• UniProt Gene Name: UBL4A
• UniProt Synonym Gene Names: DXS254E; GDX; UBL4

Uniprot Description

As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303).