EMG1 recombinant protein

EMG1, 1-244aa, Human, His tag, E Coli

Cat. Num. AAA204728

• Gene Names: EMG1; C2F; NEP1; Grcc2f
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: EMG1; 1-244aa; Human; His tag; E Coli; Ribosomal RNA small subunit methyltransferase NEP1; C2F; Grcc2f; NEP1; EMG1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI
• Sequence Length: 244

• Applicable Applications for EMG1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for EMG1 recombinant protein

EMG1 nucleolar protein homolog, also known as EMG1, is a 244 amino acid protein that is thought to be involved in ribosome biogenesis. Localized to the nucleolus, EMG1 participates in pre-18S rRNA processing and may play an important role in the assembly of the small ribosomal subunit, possibly controlling methylation during ribosome synthesis. A mutation in this gene has been associated with Bowen-Conradi syndrome. Recombinant human EMG1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for EMG1 recombinant protein

Epigenomics

References

Buchhaupt M., et al. (2001) Mol Genet Genomics. 276: 273-284; Liu P C., et al. (2001) Mol Biol Cell. 12: 3644-3657.

NCBI and Uniprot Product Information

• NCBI GI #: 194328699
• NCBI GeneID: 10436
• NCBI Accession #: NP_006322
• NCBI GenBank Nucleotide #: NM_006331.7
• UniProt Accession #: Q92979; O00675; O00726
• Molecular Weight: 29.1 kDa (267aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ribosomal RNA small subunit methyltransferase NEP1 isoform 1
• NCBI Official Synonym Full Names: EMG1, N1-specific pseudouridine methyltransferase
• NCBI Official Symbol: EMG1
• NCBI Official Synonym Symbols: C2F; NEP1; Grcc2f
• NCBI Protein Information: ribosomal RNA small subunit methyltransferase NEP1
• UniProt Protein Name: Ribosomal RNA small subunit methyltransferase NEP1
• UniProt Gene Name: EMG1

NCBI Description

This gene encodes an essential, conserved eukaryotic protein that methylates pseudouridine in 18S rRNA. The related protein in yeast is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

S-adenosyl-L-methionine-dependent pseudouridine N1-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position (PubMed:20047967). Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes ().