MuTED recombinant protein

MuTED, 1-187aa, Human, His tag, E Coli

Cat. Num. AAA204621

• Gene Names: BLOC1S5; MU; BLOS5; MUTED
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MuTED; 1-187aa; Human; His tag; E Coli; Muted homolog; Mu; MuTED recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF, 1mM EDTA
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSGGGTE TPVGCEAAPG GGSKKRDSLG TAGSAHLIIK DLGEIHSRLL DHRPVIQGET RYFVKEFEEK RGLREMRVLE NLKNMIHETN EHTLPKCRDT MRDSLSQVLQ RLQAANDSVC RLQQREQERK KIHSDHLVAS EKQHMLQWDN FMKEQPNKRA EVDEEHRKAM ERLKEQYAEM EKDLAKFSTF
• Sequence Length: 187

• Applicable Applications for MuTED recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MuTED recombinant protein

Muted homolog, also known as MuTED, is involved in the development of lysosome related organelles, such as melanosomes and platelet dense granules. MuTED interacts with pallidin, dystrobrevin binding protein 1 and CNO/cappuccino. It is part of the biogenesis of lysosome related organelles complex 1 (BLOC1). Also, MuTED is ubiquitously expressed with higher levels in brain, bone marrow, kidney, and liver and lower levels in skeletal muscle. Recombinant human MuTED protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MuTED recombinant protein

Signal Transduction

References

Ciciotte S L., et al. (2003) Blood. 101: 4402-4407; Starcevic M., et al. (2004) J Biol Chem. 279: 28393-28401.

NCBI and Uniprot Product Information

• NCBI GI #: 41152237
• NCBI GeneID: 63915
• NCBI Accession #: NP_958437
• NCBI GenBank Nucleotide #: NM_201280.2
• UniProt Accession #: Q8TDH9; Q0VDJ6; Q0VDJ7; Q5THS1; Q68D56; Q8N5F9; Q9NU16; B4DVM2
• Molecular Weight: 24 kDa (210aa), confirmed by MALDI-TOF
• NCBI Official Full Name: biogenesis of lysosome-related organelles complex 1 subunit 5 isoform 1
• NCBI Official Synonym Full Names: biogenesis of lysosomal organelles complex 1 subunit 5
• NCBI Official Symbol: BLOC1S5
• NCBI Official Synonym Symbols: MU; BLOS5; MUTED
• NCBI Protein Information: biogenesis of lysosome-related organelles complex 1 subunit 5
• UniProt Protein Name: Biogenesis of lysosome-related organelles complex 1 subunit 5
• UniProt Gene Name: BLOC1S5
• UniProt Synonym Gene Names: MUTED; BLOC-1 subunit 5

NCBI Description

This gene encodes a component of BLOC-1 (biogenesis of lysosome-related organelles complex 1). Components of this complex are involved in the biogenesis of organelles such as melanosomes and platelet-dense granules. A mouse model for Hermansky-Pudlak Syndrome is mutated in the murine version of this gene. Alternative splicing results in multiple transcript variants. Read-through transcription exists between this gene and the upstream EEF1E1 (eukaryotic translation elongation factor 1 epsilon 1) gene, as well as with the downstream TXNDC5 (thioredoxin domain containing 5) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking.