WBP2 recombinant protein

WBP2, 1-261aa, Human, His tag, E Coli

Cat. Num. AAA204288

• Gene Names: WBP2; WBP-2; GRAMD6; DFNB107
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: WBP2; 1-261aa; Human; His tag; E Coli; WW domain binding protein 2; WBP-2; WBP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMALNKNH SEGGGVIVNN TESILMSYDH VELTFNDMKN VPEAFKGTKK GTVYLTPYRV IFLSKGKDAM QSFMMPFYLM KDCEIKQPVF GANYIKGTVK AEAGGGWEGS ASYKLTFTAG GAIEFGQRML QVASQASRGE VPSGAYGYSY MPSGAYVYPP PVANGMYPCP PGYPYPPPPP EFYPGPPMMD GAMGYVQPPP PPYPGPMEPP VSGPDVPSTP AAEAKAAEAA ASAYYNPGNP HNVYMPTSQP PPPPYYPPED KKTQ
• Sequence Length: 261

• Applicable Applications for WBP2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for WBP2 recombinant protein

WBP2 (WW domain-binding protein 2) is a steroid hormone receptor coactivator. This protein contains 1 GRAM domain. The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. It binds to the WW domain of Yes kinase-associated protein (YAP1) by its PY motifs. The WW-binding 1 motif mediates interaction with NEDD4. Recombinant human WBP2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for WBP2 recombinant protein

Epigenomics

References

Chen H.I., et al. (1997) J. Biol. Chem. 272:17070-17077; Pirozzi G., et al. (1997) J. Biol. Chem. 272:14611-14616

NCBI and Uniprot Product Information

• NCBI GI #: 24430132
• NCBI GeneID: 23558
• NCBI Accession #: NP_036610
• NCBI GenBank Nucleotide #: NM_012478.3
• UniProt Accession #: Q969T9; O95638; B4DFG2
• Molecular Weight: 30.5 kDa (284aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: WW domain-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: WW domain binding protein 2
• NCBI Official Symbol: WBP2
• NCBI Official Synonym Symbols: WBP-2; GRAMD6; DFNB107
• NCBI Protein Information: WW domain-binding protein 2
• UniProt Protein Name: WW domain-binding protein 2
• Protein Family: WW domain-binding protein
• UniProt Gene Name: WBP2
• UniProt Synonym Gene Names: WBP-2

NCBI Description

The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein that is a transcriptional coactivator of estrogen receptor alpha and progesterone receptor. Defects in this gene have been associated with hearing impairment. [provided by RefSeq, Jan 2017]

Uniprot Description

Acts as transcriptional coactivator of estrogen and progesterone receptors (ESR1 and PGR) upon hormone activation (PubMed:16772533). In presence of estrogen, binds to ESR1-responsive promoters (PubMed:16772533). Required for YAP1 coactivation function on PGR activity (PubMed:16772533). Synergizes with WBP2 in enhancing PGR activity (PubMed:16772533). Modulates expression of post-synaptic scaffolding proteins via regulation of ESR1, ESR2 and PGR ().