SDS-Page
PFDN1 recombinant protein
PFDN1, 14-122aa, Human, His tag, E Coli
Gene Names
PFDN1; PDF; PFD1
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
PFDN1; 14-122aa; Human; His tag; E Coli; Prefoldin subunit 1; PDF; PFD1; PFDN1 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MTELQAKVID TQQKVKLADI QIEQLNRTKK HAHLTDTEIM TLVDETNMYE GVGRMFILQS KEAIHSQLLE KQKIAEEKIK ELEQKKSYLE RSVKEAEDNI REMLMARRAQ
Sequence Length
122
Applicable Applications for PFDN1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PFDN1 recombinant protein
PFDN1 is one of sixsubunits of prefoldin, a heterohexameric chaperone protein which assists in the correct folding of other proteins. This protein delivers nonnative target proteins, principally actins and tubulins, to the eukaryotic cytosolic chaperonin for facilitated folding. Defects in PFDN1 displayed phenotypes characteristic of defects in cytoskeletal function, including manifestations of ciliary dyskinesia, neuronal loss, and defects in B and T cell development and function. Recombinant human PFDN1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for PFDN1 recombinant protein
References
Ruley HE., et al. (2008) J Immunol. 181 (1):476-84.; Cowan NJ., et al (2004) J Biol Chem. 279 (6):4196-203.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15.0kDa (130aa), confirmed by MALDI-TOF
NCBI Official Full Name
prefoldin subunit 1
NCBI Official Synonym Full Names
prefoldin subunit 1
NCBI Official Symbol
PFDN1
NCBI Official Synonym Symbols
PDF; PFD1
NCBI Protein Information
prefoldin subunit 1
UniProt Protein Name
Prefoldin subunit 1
Protein Family
UniProt Gene Name
PFDN1
UniProt Synonym Gene Names
PFD1
NCBI Description
This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008]
Uniprot Description
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Research Articles on PFDN1
Similar Products
Product Notes
The PFDN1 pfdn1 (Catalog #AAA203820) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's PFDN1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PFDN1 pfdn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MTELQAKVID TQQKVKLADI QIEQLNRTKK HAHLTDTEIM TLVDETNMYE GVGRMFILQS KEAIHSQLLE KQKIAEEKIK ELEQKKSYLE RSVKEAEDNI REMLMARRAQ. It is sometimes possible for the material contained within the vial of "PFDN1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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