SGTA recombinant protein

SGTA, 1-313aa, Human, His tag, E Coli

Cat. Num. AAA203528

• Gene Names: SGTA; SGT; hSGT; alphaSGT
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: SGTA; 1-313aa; Human; His tag; E Coli; Small glutamine-rich tetratricopeptide repeat-containing protein alpha; alphaSGT; hSGT; SGT; Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha; uBP; Vpu binding protein.; SGTA recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MDNKKRLAYA IIQFLHDQLR HGGLSSDAQE SLEVAIQCLE TAFGVTVEDS DLALPQTLPE IFEAAATGKE MPQDLRSPAR TPPSEEDSAE AERLKTEGNE QMKVENFEAA VHFYGKAIEL NPANAVYFCN RAAAYSKLGN YAGAVQDCER AICIDPAYSK AYGRMGLALS SLNKHVEAVA YYKKALELDP DNETYKSNLK IAELKLREAP SPTGGVGSFD IAGLLNNPGF MSMASNLMNN PQIQQLMSGM ISGGNNPLGT PGTSPSQNDL ASLIQAGQQF AQQMQQQNPE LIEQLRSQIR SRTPSASNDD QQELEHHHHH H
• Sequence Length: 313

• Applicable Applications for SGTA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SGTA recombinant protein

SGTA, also known as SGT or hSGT, is a ubiquitously expressed protein that contains three TPR protein-protein interaction repeats. This protien is believed to function as a component of the androgen receptor (AR)-chaperone-cochaperone complex, acting as a cochaperone involved in androgen signaling. In addition, SGTA functions as a cochaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity. On the basis of its role in apoptosis and androgen signaling, SGTA is a potential candidate for PCOS (polycystic ovary syndrome), a disorder characterized by androgen excess, obesity and menstrual disturbances. Recombinant SGTA protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SGTA recombinant protein

Heat Shock Proteins

References

Cziepluch C., et al. (1998) J Virol. 72 (5):4149-56. ; Callahan MA., et al. (1998) J Virol. 72 (6):5189-97. ;

NCBI and Uniprot Product Information

• NCBI GI #: 4506921
• NCBI GeneID: 6449
• NCBI Accession #: NP_003012
• NCBI GenBank Nucleotide #: NM_003021.3
• UniProt Accession #: O43765; Q6FIA9; Q9BTZ9; D6W610
• Molecular Weight: 35.1 kDa (321aa), confirmed by MALDI-TOF
• NCBI Official Full Name: small glutamine-rich tetratricopeptide repeat-containing protein alpha
• NCBI Official Synonym Full Names: small glutamine rich tetratricopeptide repeat containing alpha
• NCBI Official Symbol: SGTA
• NCBI Official Synonym Symbols: SGT; hSGT; alphaSGT
• NCBI Protein Information: small glutamine-rich tetratricopeptide repeat-containing protein alpha
• UniProt Protein Name: Small glutamine-rich tetratricopeptide repeat-containing protein alpha
• Protein Family: Small glutamine-rich tetratricopeptide repeat-containing protein
• UniProt Gene Name: SGTA
• UniProt Synonym Gene Names: SGT; SGT1; UBP

NCBI Description

This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function. [provided by RefSeq, Jul 2008]

Uniprot Description

Co-chaperone that binds misfolded and hydrophobic patches-containing client proteins in the cytosol. Mediates their targeting to the endoplasmic reticulum but also regulates their sorting to the proteasome when targeting fails (PubMed:28104892). Functions in tail-anchored/type II transmembrane proteins membrane insertion constituting with ASNA1 and the BAG6 complex a targeting module (PubMed:28104892). Probably functions upstream of the BAG6 complex and ASNA1, binding more rapidly the transmembrane domain of newly synthesized proteins (PubMed:28104892). It is also involved in the regulation of the endoplasmic reticulum-associated misfolded protein catabolic process via its interaction with BAG6: collaborates with the BAG6 complex to maintain hydrophobic substrates in non-ubiquitinated states (PubMed:23129660, PubMed:25179605). Competes with RNF126 for interaction with BAG6, preventing the ubiquitination of client proteins associated with the BAG6 complex (PubMed:27193484). Binds directly to HSC70 and HSP70 and regulates their ATPase activity (PubMed:18759457).