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SDS-Page

Cell Division Cycle Protein 16 Recombinant Protein | CDC16 recombinant protein

Recombinant Cell Division Cycle Protein 16 (CDC16)

Reactivity
Human
Applications
ELISA, Immunoprecipitation, SDS-Page, Western Blot
Purity
> 90%
Synonyms
Cell Division Cycle Protein 16; Recombinant Cell Division Cycle Protein 16 (CDC16); Anaphase-Promoting Complex Subunit 6; Cyclosome subunit 6; CDC16 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Source: Prokaryotic expression
Reactivity
Human
Purity/Purification
> 90%
Form/Format
Freeze-dried powder
Sequence Length
619
Applicable Applications for CDC16 recombinant protein
SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
Usage
Reconstitute in PBS or others.
Endotoxin Level
< 1.0EU per 1ug (determined by the LAL method)
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Fragment
Met1~Ser152
Tag
Two N-terminal Tags, His-tag and T7-tag
Residues & Tags
Met1~Ser152 with N-terminal His Tag
Subcellular Location
Secreted
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
Predicted: 21.7kDa
NCBI Official Full Name
Cell division cycle protein 16
UniProt Protein Name
Cell division cycle protein 16 homolog
UniProt Gene Name
Cdc16
UniProt Synonym Gene Names
Anapc6; APC6
UniProt Entry Name
CDC16_MOUSE

Uniprot Description

CDC16: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with PPP5C and CDC20. Belongs to the APC6/CDC16 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Cellular Component: anaphase-promoting complex; centrosome; cytoplasm; nucleus; spindle microtubule

Molecular Function: protein binding

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Product Notes

The CDC16 cdc16 (Catalog #AAA2034616) is a Recombinant Protein produced from E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Cell Division Cycle Protein 16 (CDC16) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cell Division Cycle Protein 16 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CDC16 cdc16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cell Division Cycle Protein 16, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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