P-Selectin Glycoprotein Ligand 1 Recombinant Protein | PSGL1 recombinant protein

Recombinant P-Selectin Glycoprotein Ligand 1 (PSGL1)

Cat. Num. AAA2034526

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Reactivity: Rat
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: P-Selectin Glycoprotein Ligand 1; Recombinant P-Selectin Glycoprotein Ligand 1 (PSGL1); Selectin P Ligand; PSGL1 recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Rat
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 412

• Applicable Applications for PSGL1 recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Residues & Tags: N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 331284236
• NCBI GeneID: 6404
• NCBI Accession #: NP_002997.2
• NCBI GenBank Nucleotide #: NM_003006.4
• Molecular Weight: 44,648 Da
• NCBI Official Full Name: P-selectin glycoprotein ligand 1 isoform 2
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• Protein Family: P-selectin glycoprotein ligand
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: integral to membrane; integral to plasma membrane; membrane; plasma membrane; uropod

Molecular Function: protein binding

Biological Process: leukocyte adhesive activation; leukocyte migration