Cysteine Rich Secretory Protein 1 Recombinant Protein | CRISP1 recombinant protein

Recombinant Cysteine Rich Secretory Protein 1 (CRISP1)

Cat. Num. AAA2033510

• Gene Names: CRISP1; ARP; AEGL1; HUMARP; CRISP-1; HEL-S-57; HSCRISP1D; HSCRISP1G
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Cysteine Rich Secretory Protein 1; Recombinant Cysteine Rich Secretory Protein 1 (CRISP1); AEGL1; ARP; CRISP-1; HSCRISP1D; HSCRISP1G; HUMARP; Acidic Epididymal Glycoprotein-like 1; Acidic epididymal glycoprotein homolog; AEG-like protein; CRISP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: Q DSSQENRLEK LSTTKMSVQE EIVSKHNQLR RMVSPSGSDL LKMEWNYDAQ VNAQQWADKC TFSHSPIELR TTNLRCGENL FMSSYLASWS SAIQGWYNEY KDLTYDVGPK QPDSVVGHYT QVVWNSTFQV ACGVAECPKN PLRYYYVCHY CPVGNYQGRL YTPYTAGEPC ASCPDHCEDG LCTNSCGHED KYTNCKYLKK MLSCEHELLK KGCKATCLCEGKIH
• Sequence Length: 249

• Applicable Applications for CRISP1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 29.3kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Gln20~His244
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 25121982
• NCBI GeneID: 167
• NCBI Accession #: NP_001122.2
• NCBI GenBank Nucleotide #: NM_001131.2
• Molecular Weight: 20,462 Da
• NCBI Official Full Name: cysteine-rich secretory protein 1 isoform 1
• NCBI Official Synonym Full Names: cysteine rich secretory protein 1
• NCBI Official Symbol: CRISP1
• NCBI Official Synonym Symbols: ARP; AEGL1; HUMARP; CRISP-1; HEL-S-57; HSCRISP1D; HSCRISP1G
• NCBI Protein Information: cysteine-rich secretory protein 1
• UniProt Protein Name: Cysteine-rich secretory protein 1
• Protein Family: Cysteine-rich secretory protein
• UniProt Gene Name: CRISP1
• UniProt Synonym Gene Names: AEGL1; CRISP-1
• UniProt Entry Name: CRIS1_HUMAN

NCBI Description

Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head, where it plays a role in sperm-egg fusion. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

CRISP1: May have a role in sperm-egg fusion and maturation. Belongs to the CRISP family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; nucleus

Molecular Function: calcium channel regulator activity

Biological Process: binding of sperm to zona pellucida; fusion of sperm to egg plasma membrane; regulation of acrosome reaction