Molybdenum Cofactor Synthesis 1 Recombinant Protein | MOCS1 recombinant protein

Recombinant Molybdenum Cofactor Synthesis 1 (MOCS1)

Cat. Num. AAA2033364

• Gene Names: MOCS1; MIG11; MOCOD; MOCODA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Molybdenum Cofactor Synthesis 1; Recombinant Molybdenum Cofactor Synthesis 1 (MOCS1); MIG11; MOCOD; MOCS1A; MOCS1B; Molybdenum Cofactor Biosynthesis Protein 1; Cell migration-inducing gene 11 protein; Cyclic pyranopterin monophosphate synthase accessory; MOCS1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: THSK CLSTGSQAPD APSGPGPTSN QLTHVDSAGR ASMVDVGGKP ETERVAVASA MVLLGPVAFK LVQQNQLKKG DALVVAQLAG VQAAKLTSQL IPLCHHVALS HVQVHLELDS TRHAVLIQAS CRARGPTGVE MEALTSAAMA ALTVYDMCKA VSRDIVVTEV KLISKTGGQRGDFHRA
• Sequence Length: 533

• Applicable Applications for MOCS1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 22.4kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Thr457~Ala636
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 56204710
• NCBI GeneID: 4337
• UniProt Accession #: Q9NZB8; O14940; O14941; O75710; Q5J7W0; Q5TCE1; Q5TCE2; Q5TCE6; Q5TCE9; Q5TCF0; B3KPT7; B4DTP1
• Molecular Weight: 70,167 Da
• NCBI Official Full Name: molybdenum cofactor synthesis 1
• NCBI Official Synonym Full Names: molybdenum cofactor synthesis 1
• NCBI Official Symbol: MOCS1
• NCBI Official Synonym Symbols: MIG11; MOCOD; MOCODA
• NCBI Protein Information: molybdenum cofactor biosynthesis protein 1
• UniProt Protein Name: Molybdenum cofactor biosynthesis protein 1
• UniProt Gene Name: MOCS1
• UniProt Entry Name: MOCS1_HUMAN

NCBI Description

Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However, only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA, whereas additional splice variants, whose full-length natures have yet to be determined, are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency, type A. A related pseudogene has been identified on chromosome 16. [provided by RefSeq, Jan 2010]

Uniprot Description

MOCS1: Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of a guanosine derivative to precursor Z during molybdenum cofactor biosynthesis. Defects in MOCS1 are the cause of molybdenum cofactor deficiency type A (MOCOD type A); an autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.1.99.18; Enzyme, misc.

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytosol; molybdopterin synthase complex; nucleus

Molecular Function: 4 iron, 4 sulfur cluster binding; GTP binding; metal ion binding

Biological Process: Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; vitamin metabolic process; water-soluble vitamin metabolic process

Disease: Molybdenum Cofactor Deficiency, Complementation Group A