Chimerin 2 Recombinant Protein | CHN2 recombinant protein

Recombinant Chimerin 2 (CHN2)

Cat. Num. AAA2033201

• Gene Names: CHN2; BCH; CHN2-3; ARHGAP3; RHOGAP3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Chimerin 2; Recombinant Chimerin 2 (CHN2); ARHGAP3; BCH; RHoGAP3; Chimaerin; Beta-Chimaerin; Beta-chimerin; Rho GTPase-activating protein 3; CHN2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: VYCCDLTTLV KAHNTQRPMV VDICIREIEA RGLKSEGLYR VSGFTEHIED VKMAFDRDGE KADISANIYP DINIITGALK LYFRDLPIPI ITYDTYTKFI EAAKISNADE RLEAVHEVLM LLPPAHYETL RYLMIHLKKV TMNEKDNLMN AENLGIVFGP TLMRPPEDST LTTLHDMRYQ
• Sequence Length: 468

• Applicable Applications for CHN2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 24.3kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Val101~Gln280
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119614325
• NCBI GeneID: 1124
• Molecular Weight: 62,441 Da
• NCBI Official Full Name: chimerin (chimaerin) 2
• NCBI Official Synonym Full Names: chimerin 2
• NCBI Official Symbol: CHN2
• NCBI Official Synonym Symbols: BCH; CHN2-3; ARHGAP3; RHOGAP3
• NCBI Protein Information: beta-chimaerin
• UniProt Protein Name: Beta-chimaerin
• Protein Family: Beta-chimaerin
• UniProt Gene Name: CHN2
• UniProt Synonym Gene Names: ARHGAP3; BCH
• UniProt Entry Name: CHIO_HUMAN

NCBI Description

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

ARHGAP3: GTPase-activating protein for p21-rac. Insufficient expression of beta-2 chimaerin is expected to lead to higher Rac activity and could therefore play a role in the progression from low-grade to high-grade tumors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: cytoplasm; cytosol; membrane

Molecular Function: GTPase activator activity; metal ion binding; protein binding; SH3/SH2 adaptor activity

Biological Process: positive regulation of GTPase activity; positive regulation of signal transduction; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction