Killer Cell Lectin Like Receptor Subfamily K Recombinant Protein | KLRK1 recombinant protein

Recombinant Killer Cell Lectin Like Receptor Subfamily K, Member 1 (KLRK1)

Cat. Num. AAA2033078

• Gene Names: KLRK1; KLR; CD314; NKG2D; NKG2-D; D12S2489E
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Killer Cell Lectin Like Receptor Subfamily K; Recombinant Killer Cell Lectin Like Receptor Subfamily K; Member 1 (KLRK1); CD314; KLR-K1; KLR; NKG2-D; NKG2-D Type II Integral Membrane Protein; NK cell receptor D; NKG2-D-activating NK receptor; KLRK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: AVFITL LCNKEVSVSS REGYCGPCPNDWICHRNNCY QFFNENKAWN QSQASCLSQN SSLLKIYSKE EQDFLKLVKSYHWMGLVQSP ANGSWQWEDG SSLSPNELTL VKTPSGTCAV YGSSFKAYTEDCSNPNTYIC MKRAV
• Sequence Length: 216

• Applicable Applications for KLRK1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 19.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Ala75~Val215
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 24980985
• NCBI GeneID: 22914
• Molecular Weight: 25,274 Da
• NCBI Official Full Name: Killer cell lectin-like receptor subfamily K, member 1
• NCBI Official Synonym Full Names: killer cell lectin like receptor K1
• NCBI Official Symbol: KLRK1
• NCBI Official Synonym Symbols: KLR; CD314; NKG2D; NKG2-D; D12S2489E
• NCBI Protein Information: NKG2-D type II integral membrane protein
• UniProt Protein Name: NKG2-D type II integral membrane protein
• Protein Family: NKG2-D type II integral membrane protein
• UniProt Gene Name: KLRK1
• UniProt Synonym Gene Names: D12S2489E; NKG2D
• UniProt Entry Name: NKG2D_HUMAN

NCBI Description

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster. [provided by RefSeq, Dec 2010]

Uniprot Description

KLRK1: Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Cell surface

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: cell surface; external side of plasma membrane; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: carbohydrate binding; kinase binding; MHC class I protein binding; MHC class Ib receptor activity; protein binding; protein homodimerization activity; receptor activity

Biological Process: adaptive immune response; cell differentiation; defense response to Gram-positive bacterium; innate immune response; natural killer cell activation; positive regulation of apoptosis; positive regulation of interferon-gamma production; positive regulation of myeloid dendritic cell activation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of nitric oxide biosynthetic process; regulation of immune response; signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell costimulation