Amphiphysin Recombinant Protein | AMPH recombinant protein

Recombinant Amphiphysin (AMPH)

Cat. Num. AAA2033001

• Gene Names: AMPH; AMPH1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Amphiphysin; Recombinant Amphiphysin (AMPH); AMPH1; Stiff-Man Syndrome With Breast Cancer 128kDa Autoantigen; AMPH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: VLQKLGK ADETKDEQFE EYVQNFKRQE AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVGFYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD
• Sequence Length: 695

• Applicable Applications for AMPH recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 29.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Val24~Asp240
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 550450
• NCBI GeneID: 273
• Molecular Weight: 71,929 Da
• NCBI Official Full Name: amphiphysin
• NCBI Official Synonym Full Names: amphiphysin
• NCBI Official Symbol: AMPH
• NCBI Official Synonym Symbols: AMPH1
• NCBI Protein Information: amphiphysin
• UniProt Protein Name: Amphiphysin
• Protein Family: Amphiphysin
• UniProt Gene Name: AMPH
• UniProt Synonym Gene Names: AMPH1
• UniProt Entry Name: AMPH_HUMAN

NCBI Description

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]

Uniprot Description

amphiphysin: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: actin cytoskeleton; cell junction; synaptic vesicle; synaptic vesicle membrane; trans-Golgi network

Molecular Function: ARF guanyl-nucleotide exchange factor activity; phospholipid binding; protein binding

Biological Process: endocytosis; learning; positive regulation of GTPase activity; synaptic transmission; synaptic vesicle endocytosis