Western Blot (WB)
(Western Blot: Sample: Recombinant bACE1, Human.)
Rabbit anti-Human Beta-Site APP Cleaving Enzyme 1 Polyclonal Antibody | anti-bACE1 antibody
Beta-Site APP Cleaving Enzyme 1 (bACE1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant bACE1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant bACE1, Human.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]
Uniprot Description
BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.
Protein type: EC 3.4.23.46; Membrane protein, integral; Protease
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: axon; cell surface; cytoplasmic vesicle membrane; endoplasmic reticulum lumen; endosome; endosome membrane; Golgi apparatus; integral component of membrane; integral component of plasma membrane; late endosome; membrane raft; multivesicular body; plasma membrane; trans-Golgi network
Molecular Function: amyloid-beta binding; aspartic-type endopeptidase activity; beta-aspartyl-peptidase activity; enzyme binding; peptidase activity; protein binding
Biological Process: beta-amyloid metabolic process; cellular protein metabolic process; membrane protein ectodomain proteolysis; protein catabolic process; proteolysis; regulation of synaptic transmission
Research Articles on bACE1
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Product Notes
The bACE1 bace1 (Catalog #AAA2032850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Beta-Site APP Cleaving Enzyme 1 (bACE1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-Site APP Cleaving Enzyme 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the bACE1 bace1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-Site APP Cleaving Enzyme 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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