Rabbit anti-Human TATA Box Binding Protein Associated Factor 2 Polyclonal Antibody | anti-TAF2 antibody

TATA Box Binding Protein Associated Factor 2 (TAF2) Polyclonal Antibody

Cat. Num. AAA2032837

• Gene Names: TAF2; MRT40; TAF2B; CIF150; TAFII150
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: TATA Box Binding Protein Associated Factor 2; Polyclonal Antibody; TATA Box Binding Protein Associated Factor 2 (TAF2) Polyclonal Antibody; CIF150; TAF2B; TAFII150; TAF2 RNA Polymerase II; Transcription Initiation Factor TFIID Subunit 2; 150 kDa cofactor of initiator function RNA polymerase II TBP-associated factor B; anti-TAF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against TAF2. It has been selected for its ability to recognize TAF2 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 1199

• Applicable Applications for anti-TAF2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western Blot: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunoglobulin Type: IgG
• Conjugate: No Conjugate
• Cross Reactivity: Human
• Organism Species: Human
• Fragment: TAF2 (Pro918~Asp1199)
• Quality Control: Content: The quality control contains recombinant TAF2 (Pro918~Asp1199) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2057796
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TAF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TAF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 4507347
• NCBI GeneID: 6873
• NCBI Accession #: NP_003175.1
• NCBI GenBank Nucleotide #: NM_003184.3
• UniProt Accession #: Q6P1X5; O43487; O43604; O60668; Q86WW7; Q8IWK4; B2RE82
• Molecular Weight: 136,971 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 2
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 2
• NCBI Official Symbol: TAF2
• NCBI Official Synonym Symbols: MRT40; TAF2B; CIF150; TAFII150
• NCBI Protein Information: transcription initiation factor TFIID subunit 2
• UniProt Protein Name: Transcription initiation factor TFIID subunit 2
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF2
• UniProt Synonym Gene Names: CIF150; TAF2B; TAF(II)150; TAFII-150; TAFII150

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF2: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter. Belongs to the TAF2 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: chromatin binding; protein binding; sequence-specific DNA binding; transcription regulatory region DNA binding

Biological Process: G2/M transition of mitotic cell cycle; positive regulation of transcription from RNA polymerase II promoter; response to organic cyclic compound; RNA polymerase II transcriptional preinitiation complex assembly; transcription elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

Disease: Mental Retardation, Autosomal Recessive 40