Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PCM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Pericentriolar Material 1 Polyclonal Antibody | anti-PCM1 antibody
Pericentriolar Material 1 (PCM1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PCM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PCM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
Uniprot Description
PCM-1: a pericentriolar protein of unknown function.
Protein type: Cell cycle regulation; Oncoprotein
Chromosomal Location of Human Ortholog: 8p22
Cellular Component: apical part of cell; centriole; centrosome; cytoplasm; cytosol; membrane; nuclear membrane; pericentriolar material; protein complex
Molecular Function: identical protein binding; protein binding
Biological Process: centrosome cycle; cytoplasmic microtubule organization; G2/M transition of mitotic cell cycle; interkinetic nuclear migration; negative regulation of neurogenesis; neuron migration; non-motile cilium assembly; positive regulation of intracellular protein transport; protein localization to centrosome; regulation of G2/M transition of mitotic cell cycle; social behavior
Disease: Thyroid Carcinoma, Papillary
Research Articles on PCM1
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Product Notes
The PCM1 pcm1 (Catalog #AAA2032835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pericentriolar Material 1 (PCM1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pericentriolar Material 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PCM1 pcm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pericentriolar Material 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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