Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PCYOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Prenylcysteine Oxidase 1 Polyclonal Antibody | anti-PCYOX1 antibody
Prenylcysteine Oxidase 1 (PCYOX1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PCYOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PCYOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PCYOX1 knockout Hela cell lysate; Predicted MW: 49,57kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human PCYOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PCYOX1 knockout Hela cell lysate; Predicted MW: 49,57kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human PCYOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
Prenylcysteine is released during the degradation of prenylated proteins. PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product (Tschantz et al., 1999 [PubMed 10585463]).[supplied by OMIM, Mar 2008]
Uniprot Description
PCYOX1: Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. Belongs to the prenylcysteine oxidase family.
Protein type: EC 1.8.3.5; Oxidoreductase; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: lysosome; plasma membrane; vacuolar membrane
Molecular Function: chloride-transporting ATPase activity; prenylcysteine oxidase activity
Biological Process: prenylated protein catabolic process; prenylcysteine catabolic process; prenylcysteine metabolic process
Research Articles on PCYOX1
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Product Notes
The PCYOX1 pcyox1 (Catalog #AAA2032714) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Prenylcysteine Oxidase 1 (PCYOX1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Prenylcysteine Oxidase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PCYOX1 pcyox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prenylcysteine Oxidase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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