Rabbit anti-Human Cadherin 5 Polyclonal Antibody | anti-CDH5 antibody

Cadherin 5 (CDH5) Polyclonal Antibody

Cat. Num. AAA2032550

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Cadherin 5; Polyclonal Antibody; Cadherin 5 (CDH5) Polyclonal Antibody; CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin; Vascular Endothelial; anti-CDH5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against CDH5. It has been selected for its ability to recognize CDH5 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 784

• Applicable Applications for anti-CDH5 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western Blot: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunoglobulin Type: IgG
• Conjugate: No Conjugate
• Cross Reactivity: Human, Bovine
• Organism Species: Human
• Fragment: CDH5 (Gly69~Gly391)
• Quality Control: Content: The quality control contains recombinant CDH5 (Gly69~Gly391) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2054369
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Bovine Kidney Tissue; Lane2: Bovine Brain Tissue; Lane3: Bovine Spleen Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant CDH5, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Lung Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Skin Cancer Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 166362713
• NCBI GeneID: 1003
• NCBI Accession #: NP_001786.2
• NCBI GenBank Nucleotide #: NM_001795.4
• UniProt Accession #: P33151; Q4VAI5; Q4VAI6
• Molecular Weight: 74,499 Da
• NCBI Official Full Name: cadherin-5 preproprotein
• NCBI Official Synonym Full Names: cadherin 5
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: bicellular tight junction; cell junction; cell surface; cell-cell adherens junction; external side of plasma membrane; integral component of membrane; intercellular junction; membrane; plasma membrane

Molecular Function: beta-catenin binding; calcium ion binding; ion channel binding; protein binding; protein phosphatase binding; receptor binding

Biological Process: blood vessel maturation; homophilic cell adhesion; intercellular junction assembly; negative regulation of cell proliferation; regulation of establishment of cell polarity; transforming growth factor beta receptor signaling pathway