Western Blot (WB)
(Western Blot: Sample: Rat Brain Tissue.)
Rabbit anti-Human V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 Polyclonal Antibody | anti-ErbB4 antibody
V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Rat Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ErbB4, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ErbB4, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.
Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group
Chromosomal Location of Human Ortholog: 2q34
Cellular Component: basolateral plasma membrane; cytosol; extracellular region; integral component of membrane; mitochondrial matrix; mitochondrion; nucleoplasm; nucleus; plasma membrane; receptor complex
Molecular Function: ATP binding; epidermal growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein homodimerization activity; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; signal transducer, downstream of receptor, with protein tyrosine kinase activity; transcription regulatory region DNA binding; transmembrane receptor protein tyrosine kinase activity
Biological Process: cell fate commitment; cell migration; cell proliferation; cellular response to epidermal growth factor stimulus; central nervous system morphogenesis; embryonic pattern specification; ERBB2 signaling pathway; heart development; lactation; MAPK cascade; mitochondrial fragmentation involved in apoptotic process; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of ERBB signaling pathway; negative regulation of neuron migration; nervous system development; neural crest cell migration; olfactory bulb interneuron differentiation; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; phosphatidylinositol phosphorylation; positive regulation of apoptosis; positive regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling; positive regulation of protein localization to cell surface; positive regulation of protein phosphorylation; positive regulation of STAT protein import into nucleus; positive regulation of transcription, DNA-templated; positive regulation of tyrosine phosphorylation of STAT protein; protein autophosphorylation; regulation of cell migration; regulation of cell motility; regulation of molecular function; signal transduction; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Amyotrophic Lateral Sclerosis 19
Research Articles on ErbB4
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Product Notes
The ErbB4 erbb4 (Catalog #AAA2032458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The V-Erb An Erythroblastic Leukemia Viral Oncogene Homolog 4 (ErbB4) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's V-Erb An Erythroblastic Leukemia Viral Oncogene Homolog 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ErbB4 erbb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V-Erb A Erythroblastic Leukemia Viral Oncogene Homolog 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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