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Western Blot (WB) (Western Blot Lane1: Human Lung Tissue Lane2: Porcine Muscle Tissue Lane3: Porcine Heart Tissue Primary Ab: 1ug/mL Rabbit Anti-Human HSP27 Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )

Mouse anti-Human Heat Shock 27kDa Protein 1 Monoclonal Antibody | anti-HSPB1 antibody

Heat Shock 27kDa Protein 1 (HSPB1) Monoclonal Antibody

Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Heat Shock 27kDa Protein 1; Monoclonal Antibody; Heat Shock 27kDa Protein 1 (HSPB1) Monoclonal Antibody; HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;; anti-HSPB1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
D2
Specificity
The antibody is a mouse monoclonal antibody raised against HSPB1. It has been selected for its ability to recognize HSPB1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Length
205
Applicable Applications for anti-HSPB1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:100-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Pig
Organism Species
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2046186
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot Lane1: Human Lung Tissue Lane2: Porcine Muscle Tissue Lane3: Porcine Heart Tissue Primary Ab: 1ug/mL Rabbit Anti-Human HSP27 Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )

Western Blot (WB) (Western Blot Lane1: Human Lung Tissue Lane2: Porcine Muscle Tissue Lane3: Porcine Heart Tissue Primary Ab: 1ug/mL Rabbit Anti-Human HSP27 Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P Samples:Human Stomach Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P Samples:Human Stomach Tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Human Kidney Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples:Human Kidney Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P Samples:Human Brain Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P Samples:Human Brain Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,783 Da
NCBI Official Full Name
heat shock protein beta-1
NCBI Official Synonym Full Names
heat shock protein family B (small) member 1
NCBI Official Symbol
HSPB1
NCBI Official Synonym Symbols
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
NCBI Protein Information
heat shock protein beta-1
UniProt Protein Name
Heat shock protein beta-1
Protein Family
UniProt Gene Name
HSPB1
UniProt Synonym Gene Names
HSP27; HSP28; HspB1; HSP 27; SRP27

NCBI Description

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Uniprot Description

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Research Articles on HSPB1

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Product Notes

The HSPB1 hspb1 (Catalog #AAA2032033) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Heat Shock 27kDa Protein 1 (HSPB1) Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock 27kDa Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:100-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HSPB1 hspb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock 27kDa Protein 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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