SDS-Page
RheB recombinant protein
RheB, 1-181aa, Human,T7-tag, E Coli
Gene Names
RHEB; RHEB2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
RheB; 1-181aa; Human; T7-tag; E Coli; Ras homolog enriched in brain; RHEB2; GTP-binding protein Rheb; Ras homolog enriched in brain GTP binding protein Rheb; MGC111559; Ras homolog enriched in brain 2; RHEB 2.; RheB recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 20mM Tris pH 8.0, 1mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MASMTGGQQM GRGSASMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYILV YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC
Sequence Length
184
Applicable Applications for RheB recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
T7 tag
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for RheB recombinant protein
Ras homolog enriched in brain (RheB) is a member of the Ras superfamily that was originally identified as an immediate-early gene in brain but is also widely expressed in other tissues. RheB encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RheB is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. Recombinant human RheB protein, fused to T7-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for RheB recombinant protein
References
Yamagata K., et al. (1994) J Biol Chem. 269 (23) 16333-9.; Tabancay AP Jr., et al. (2003). J Biol Chem. 278 (41):39921-30.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21.7 kDa (197 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
GTP-binding protein Rheb
NCBI Official Synonym Full Names
Ras homolog, mTORC1 binding
NCBI Official Symbol
RHEB
NCBI Official Synonym Symbols
RHEB2
NCBI Protein Information
GTP-binding protein Rheb
UniProt Protein Name
GTP-binding protein Rheb
Protein Family
UniProt Gene Name
RHEB
UniProt Synonym Gene Names
RHEB2
NCBI Description
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]
Uniprot Description
Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.
Research Articles on RheB
Similar Products
Product Notes
The RheB rheb (Catalog #AAA203203) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is T7 tag. AAA Biotech's RheB can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RheB rheb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MASMTGGQQM GRGSASMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYILV YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC. It is sometimes possible for the material contained within the vial of "RheB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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