HSP47 recombinant protein

HSP47 (Colligin), 18-418aa, Human, His tag, E Coli

Cat. Num. AAA203189

• Gene Names: SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: HSP47; HSP47 (Colligin); 18-418aa; Human; His tag; E Coli; Collagen-binding protein; Serpin H1; SERPINH1; RA-A47; AsTP3; Heme oxygenase 1; Collogin; 47 kDa heat shock protein; 47 kDa heat shock protein precursor; Arsenic transactivated protein 3; AsTP 3; CBP 1; CBP 2; CBP1; CBP2; Collagen binding protein; SERPINH2; Collagen binding protein 1; Collagen binding protein 2; Colligen; Colligin 1; Colligin 2; gp46; Heat shock protein 47; HGNC 1547; Hsp 47; J6; PIG 14; PIG14; PPROM; Proliferation inducing gene 14; Proliferation inducing gene 14 protein; RA A47; Rheumatoid arthritis antigen A 47; Rheumatoid arthritis related antigen RA A47; Serine or cysteine proteinase inhibitor clade H member 1; Serine or cysteine proteinase inhibitor clade H member 2; Serpin peptidase inhibitor clade H member 1.; HSP47 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 18-418aa
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL

• Applicable Applications for HSP47 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Ships on dry ice. Can be stored at +2 to +8 degree C (1 weeks). For long term storage, aliquot and store at -20 degree C or -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for HSP47 recombinant protein

Description: Heat shock protein 47 (HSP47), also known as Colligin or Serpin H1, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein acts as a molecular chaperone facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Recombinant human HSP47/Colligin protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for HSP47 recombinant protein

Heat Shock Proteins

References

Mohammed Tasab., et al. (2000) The EMBO Journal. 19 (10): 2204-2211; Maloney A., et al. (2007) Cancer Res. 67 (7):3239-53;

NCBI and Uniprot Product Information

• NCBI GI #: 32454741
• NCBI GeneID: 871
• NCBI Accession #: NP_001226
• NCBI GenBank Nucleotide #: NM_001235.3
• UniProt Accession #: P50454; P29043; Q5XPB4; Q6NSJ6; Q8IY96; Q9NP88; B3KVJ3
• Molecular Weight: 48.9 kDa (439aa), confirmed by MALDI-TOF
• NCBI Official Full Name: serpin H1
• NCBI Official Synonym Full Names: serpin family H member 1
• NCBI Official Symbol: SERPINH1
• NCBI Official Synonym Symbols: CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
• NCBI Protein Information: serpin H1
• UniProt Protein Name: Serpin H1
• UniProt Gene Name: SERPINH1
• UniProt Synonym Gene Names: CBP1; CBP2; HSP47; SERPINH2; AsTP3; Colligin

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Uniprot Description

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.