Human Constitutive Androstane Receptor ELISA Kit | CAR elisa kit

Human Constitutive Androstane Receptor (CAR) ELISA Kit

Cat. Num. AAA2031878

• Gene Names: NR1I3; CAR; CAR1; MB67
• Reactivity: Human
• Synonyms: Constitutive Androstane Receptor; Human Constitutive Androstane Receptor (CAR) ELISA Kit; NR1I3; NR1-I3; CAR1; MB67; Nuclear Receptor Subfamily 1; Group I; Member 3; Constitutive active response; Constitutive activator of retinoid response; Orphan nuclear receptor MB67; CAR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Constitutive Androstane Receptor (CAR).; No significant cross-reactivity or interference between Constitutive Androstane Receptor (CAR) and analogues was observed.
• Sequence Length: 309

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Constitutive Androstane Receptor (CAR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Constitutive Androstane Receptor (CAR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for CAR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Constitutive Androstane Receptor (CAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Constitutive Androstane Receptor (CAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Constitutive Androstane Receptor (CAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Constitutive Androstane Receptor (CAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CAR elisa kit

Signal transduction; Metabolic pathway; Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 216409712
• NCBI GeneID: 9970
• Molecular Weight: 30,262 Da
• NCBI Official Full Name: constitutive androstane receptor
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1 group I member 3
• NCBI Official Symbol: NR1I3
• NCBI Official Synonym Symbols: CAR; CAR1; MB67
• NCBI Protein Information: nuclear receptor subfamily 1 group I member 3
• UniProt Protein Name: Nuclear receptor subfamily 1 group I member 3
• Protein Family: Carboxylic acid reductase
• UniProt Gene Name: NR1I3
• UniProt Synonym Gene Names: CAR; Constitutive active response; CAR
• UniProt Entry Name: NR1I3_HUMAN

NCBI Description

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; nucleoplasm; nucleus

Molecular Function: androgen receptor activity; DNA binding; ligand-dependent nuclear receptor activity; thyroid hormone receptor activity; transcription coactivator activity; transcription factor activity; zinc ion binding

Biological Process: androgen receptor signaling pathway; gene expression; intracellular receptor-mediated signaling pathway; negative regulation of transcription, DNA-dependent; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; signal transduction; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter