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Crystallin alpha B Recombinant Protein | Cryab recombinant protein

Crystallin alpha B, 1-175aa, Mouse, E Coli

Gene Names
Cryab; P23; Crya2; HspB5; Crya-2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Crystallin alpha B; 1-175aa; Mouse; E Coli; Crya2; Crya-2; Cryab; ; Cryab recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 20mM Tris pH 8.0 and 10 % glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK
Sequence Length
175
Applicable Applications for Cryab recombinant protein
SDS-PAGE
Antigen Species
Mouse
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for Cryab recombinant protein
Alpha crystallins are composed of two gene products, alpha-A and alpha-B for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. CRYAB is expressed widely in many tissues and organs. Recombinant CRYAB was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for Cryab recombinant protein
References
Hasan, A., et al. (2002) Biochemistry 41 (52) 15876-15882; Kamradt,M.C., et al. (2002) J. Biol.Chem. 277 (41) 38731-38736 ; Reddy, G.B., et al. (2002) FEBS lett. 522 (1-3) 59-64 ; Ito, H., et al. (2002) J.Biochem.131 (4) 593-603;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20kDa (175aa), confirmed by MALDI-TOF.
NCBI Official Full Name
alpha-crystallin B chain
NCBI Official Synonym Full Names
crystallin, alpha B
NCBI Official Symbol
Cryab
NCBI Official Synonym Symbols
P23; Crya2; HspB5; Crya-2
NCBI Protein Information
alpha-crystallin B chain
UniProt Protein Name
Alpha-crystallin B chain
Protein Family
UniProt Gene Name
Cryab
UniProt Synonym Gene Names
Crya2

NCBI Description

This gene encodes a member of the small heat-shock protein (HSP20) family. The encoded protein is a molecular chaperone that protects proteins against thermal denaturation and other stresses. This protein is a component of the eye lens, regulates lens differentiation and functions as a refractive element in the lens. This protein is a negative regulator of inflammation, has anti-apoptotic properties and also plays a role in the formation of muscular tissue. Mice lacking this gene exhibit worse experimental autoimmune encephalomyelitis and inflammation of the central nervous system compared to the wild type. In mouse models, this gene has a critical role in alleviating the pathology of the neurodegenerative Alexander disease. Mutations in the human gene are associated with myofibrillar myopathy 2, fatal infantile hypertonic myofibrillar myopathy, multiple types of cataract and dilated cardiomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.

Research Articles on Cryab

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Product Notes

The Cryab cryab (Catalog #AAA203184) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Crystallin alpha B can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Cryab cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK. It is sometimes possible for the material contained within the vial of "Crystallin alpha B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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