SDS-Page
Involucrin Recombinant Protein | iNV recombinant protein
Recombinant Involucrin (iNV)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Involucrin; Recombinant Involucrin (iNV); iNV recombinant protein
Host
Host: E Coli
Source: Prokaryotic expression
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
QPTPL PPPCQKVPVE LPVEVPSKQE EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK AENPEQQLKQ EKTQRDQQLN KQLEEEKKLL DQQLDQELVKRDEQLGMKKE QL
QPTPL PPPCQKVPVE LPVEVPSKQE EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK AENPEQQLKQ EKTQRDQQLN KQLEEEKKLL DQQLDQELVKRDEQLGMKKE QL
Sequence Length
585
Applicable Applications for iNV recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
17.7kDa
Accurate Molecular Mass (KD)
26kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag, T7 tag
Organism Species
Homo sapiens (Human)
Fragment
Pro25~Phe204
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,479 Da
NCBI Official Full Name
involucrin
NCBI Official Synonym Full Names
involucrin
NCBI Official Symbol
IVL
NCBI Protein Information
involucrin
UniProt Protein Name
Involucrin
Protein Family
UniProt Gene Name
IVL
UniProt Entry Name
INVO_HUMAN
NCBI Description
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
Uniprot Description
IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family.
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: cytoplasm; cornified envelope
Molecular Function: protein binding, bridging; structural molecule activity
Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B
Research Articles on iNV
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Product Notes
The iNV ivl (Catalog #AAA2031805) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Involucrin can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the iNV ivl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.QPTPL PPPCQKVPVE LPVEVPSKQE EKHMTAVKGL PEQECEQQQK EPQEQELQQQ HWEQHEEYQK AENPEQQLKQ EKTQRDQQLN KQLEEEKKLL DQQLDQELVK RDEQLGMKKE QL. It is sometimes possible for the material contained within the vial of "Involucrin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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