Intestinal Cell Kinase Recombinant Protein | ICK recombinant protein

Recombinant Intestinal Cell Kinase (ICK)

Cat. Num. AAA2031800

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Intestinal Cell Kinase; Recombinant Intestinal Cell Kinase (ICK); ICK recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MNRYTTIKQL GDGTYGSVLL GRSIESGELI AIKKMKRKFY SWEECMNLREVKSLKKLNHA NIVKLKEVIR ENDHLYFIFE YMKENLYQLI KERNKLFPESAIRNIMYQIL QGLAFIHKHG FFHRDLKPEN LLCMGPELVK IADFGLAREIRSRPPYTDYV STRWYRAPEV LLRSTNYSSP IDIWAVGCIM AEVYTLRPLFPGASEIDTIF KICQVLGTPK KTDWPEGYQL SSAMNFLWPQ CIPNNLKTLIPNASSEAIQL LRDLLQWDPK KRPTASQALR YPYF
• Sequence Length: 632

• Applicable Applications for ICK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 36.9kDa
• Accurate Molecular Mass (KD): 34kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met1~Phe284
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 7141298
• Molecular Weight: 34,071 Da
• NCBI Official Full Name: intestinal cell kinase
• UniProt Protein Name: Serine/threonine-protein kinase ICK
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ICK
• UniProt Synonym Gene Names: KIAA0936; hICK; LCK2; MRK
• UniProt Entry Name: ICK_HUMAN

Uniprot Description

ICK: a CMGC kinase of the RCK family. May play a role in cardiac development. Expressed in heart, brain, placenta, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Also expressed in many cancer cell lines. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, CMGC; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; RCK family

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; cyclin-dependent protein kinase activity; magnesium ion binding; ATP binding

Biological Process: regulation of cell cycle; multicellular organismal development; intraflagellar transport; cilium biogenesis; signal transduction; protein amino acid phosphorylation

Disease: Endocrine-cerebroosteodysplasia