Serum Response Factor Recombinant Protein | SRF recombinant protein

Recombinant Serum Response Factor (SRF)

Cat. Num. AAA2031519

• Gene Names: Srf; AW049942; AW240594
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Serum Response Factor; Recombinant Serum Response Factor (SRF); SRF recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.9 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TTDQRMSATG FEETDLTYQV SESDSSGETK DTLKPAFTVT NLPGTTSTIQ TAPSTSTTMQ VSSGPSFPIT NYLAPVSASV SPSAVSSANG TVLKSTGSGP VSSGGLMQLP TSFTLMPGGA VAQQVPVQAI QVHQAPQQAS PSRDSSTDLT QTSSSGTVTL PATIMTSSVP TTVGGHMMYP SPHAVMYAPT SGLGDGSLTVLNAFSQAPST MQVSHSQVQE PGGVPQVFLT ASSGTVQIPV SAVQ
• Sequence Length: 504

• Applicable Applications for SRF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 28.5kDa
• Accurate Molecular Mass (KD): 36kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Thr231~Gln474
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 10048414
• NCBI GeneID: 20807
• NCBI Accession #: NP_065239.1
• NCBI GenBank Nucleotide #: NM_020493.2
• Molecular Weight: 51,247 Da
• NCBI Official Full Name: serum response factor
• NCBI Official Synonym Full Names: serum response factor
• NCBI Official Symbol: Srf
• NCBI Official Synonym Symbols: AW049942; AW240594
• NCBI Protein Information: serum response factor
• UniProt Protein Name: Serum response factor
• Protein Family: Serum response factor
• UniProt Gene Name: Srf
• UniProt Synonym Gene Names: SRF
• UniProt Entry Name: SRF_MOUSE

Uniprot Description

SRF: a transcription factor of the MADS domain family that binds to the serum response element (SRE). Regulates the transcription of immediate early genes including c-fos. Binds DNA as a multimer, probably a dimer.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Cellular Component: cell; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; chromatin DNA binding; histone deacetylase binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: developmental growth; positive regulation of axon extension; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; gastrulation; muscle maintenance; cell-cell adhesion; tangential migration from the subventricular zone to the olfactory bulb; morphogenesis of an epithelial sheet; neurite development; platelet activation; skin morphogenesis; transcription, DNA-dependent; leukocyte differentiation; actin filament organization; trophectodermal cell differentiation; regulation of water loss via skin; patterning of blood vessels; cell migration during sprouting angiogenesis; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cell differentiation; actin cytoskeleton organization and biogenesis; transcription from RNA polymerase II promoter; erythrocyte development; positive regulation of transcription by glucose; cell-matrix adhesion; response to hormone stimulus; neuron migration; cardiac myofibril assembly; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of smooth muscle contraction; heart looping; negative regulation of cell migration; associative learning; positive thymic T cell selection; regulation of cell adhesion; positive regulation of filopodium formation; in utero embryonic development; hippocampus development; sarcomere organization; mRNA transcription from RNA polymerase II promoter; contractile actin filament bundle formation; long-term memory; response to cytokine stimulus; stress fiber formation; platelet formation