Activating Transcription Factor 1 Recombinant Protein | ATF1 recombinant protein

Recombinant Activating Transcription Factor 1 (ATF1)

Cat. Num. AAA2031173

• Gene Names: ATF1; TREB36; EWS-ATF1; FUS/ATF-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Activating Transcription Factor 1; Recombinant Activating Transcription Factor 1 (ATF1); ATF1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.5 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EDSHKSNTS ETASQPGSTV PGIISQIVHQ VSSLSESEES QDSSDSIGSS QKAHGILARR PSYRKILKDL SSEDTRGRKG DGENPSISAV TSMSVPTPIY QTSSGQYIAI APNGALQLAS PGPDGVQALQ TLTMTNSSSA QQGAILQYAQ TSDGQQILVP SNQVVVQTAS GDMQTYQIRT TPSATSLPQT MVMTSPVTLTSQTTKTDDPQ LKREIRLMKN REAARECRRK KKEYVKCLEN
• Sequence Length: 271

• Applicable Applications for ATF1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 29.5kDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Glu2~Asn240
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119578555
• NCBI GeneID: 466
• Molecular Weight: 15,295 Da
• NCBI Official Full Name: activating transcription factor 1
• NCBI Official Synonym Full Names: activating transcription factor 1
• NCBI Official Symbol: ATF1
• NCBI Official Synonym Symbols: TREB36; EWS-ATF1; FUS/ATF-1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-1
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-1
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: ATF1
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-1
• UniProt Entry Name: ATF1_HUMAN

NCBI Description

This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]

Uniprot Description

ATF-1: a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation. Plays an important role in the trans-activation of the MHC class II trans-activator (CIITA) promoter III in B cells.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; protein complex binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; cellular protein complex assembly; toll-like receptor signaling pathway; response to cobalt ion; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication