Glutamate Decarboxylase 1 Recombinant Protein | GAD1 recombinant protein

Recombinant Glutamate Decarboxylase 1, Brain (GAD1)

Cat. Num. AAA2030436

• Gene Names: GAD; GAD1; glutamate decarboxylase; GLUTAMATE DECARBOXYLASE; GLUTAMATE DECARBOXYLASE 1; MKP11.30; MKP11_30
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Glutamate Decarboxylase 1; Recombinant Glutamate Decarboxylase 1; Brain (GAD1); GAD1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.7 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL QRTNSLEEKS RLVSAFRERQ SSKNLLSCENSDQGARFRRT ETDFSNL
• Sequence Length: 502

• Applicable Applications for GAD1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 42.6kDa
• Accurate Molecular Mass (KD): 40kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met1~Leu97
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 15237949
• NCBI GeneID: 831599
• NCBI Accession #: NP_197235.1
• NCBI GenBank Nucleotide #: NM_121739.3
• Molecular Weight: 57,066 Da
• NCBI Official Full Name: glutamate decarboxylase 1
• NCBI Official Symbol: GAD
• NCBI Official Synonym Symbols: GAD1; glutamate decarboxylase; GLUTAMATE DECARBOXYLASE; GLUTAMATE DECARBOXYLASE 1; MKP11.30; MKP11_30
• NCBI Protein Information: glutamate decarboxylase 1
• UniProt Protein Name: Glutamate decarboxylase 1
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: GAD1
• UniProt Synonym Gene Names: GAD; GDH1; GAD 1
• UniProt Entry Name: DCE1_ARATH

Uniprot Description

Catalyzes the production of GABA. The calmodulin-binding is calcium-dependent and it is proposed that this may, directly or indirectly, form a calcium regulated control of GABA biosynthesis.