DnaJ recombinant protein

DnaJ, 1-376aa, E Coli, E Coli

Cat. Num. AAA203031

• Gene Names: dnaJ; ECK0015; faa; groP; grpC; JW0014
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: DnaJ; 1-376aa; E Coli; DNAJA1; DNAJ2; HDJ2; HSJ2; HSPF4; DnaJ (1-376aa); Heat shock 40 kDa protein 4; DnaJ protein homolog 2; HDJ-2; HSJ-2; HSDJ; DnaJ homolog subfamily A member 1; DnaJ recombinant protein

• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 25 mM Tris-HCl Buffer (pH 8.8) containing 5 mM DTT, 100 mM NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-376aa
• Sequence: MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR

• Applicable Applications for DnaJ recombinant protein: SDS-PAGE
• Tag: Non-Tagged
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freexing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for DnaJ recombinant protein

DnaJ, Heat shock protein, functions in association with DnaK (Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70.. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues. DnaJ (amino acids 1-376) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.

Product Categories/Family for DnaJ recombinant protein

Heat Shock Proteins

References

Bardwell,J.C., et al (1986) J.Biol.Chem.261 (4);1782-1785; Ohki M.,et al (1986) J.Biol.Chem.261 (4);1778-1781; Zylicz M., et.al. (1989) EMBO J 8 (5)1601-8 ; Landry SJ., et al. (2003) Biochemistry 42 (17)4926-36;

NCBI and Uniprot Product Information

• NCBI GI #: 16128009
• NCBI GeneID: 944753
• NCBI Accession #: NP_414556
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P08622
• Molecular Weight: 41.1kDa (376aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: chaperone Hsp40, DnaK co-chaperone
• NCBI Official Symbol: dnaJ
• NCBI Official Synonym Symbols: ECK0015; faa; groP; grpC; JW0014
• NCBI Protein Information: chaperone Hsp40, DnaK co-chaperone
• UniProt Protein Name: Chaperone protein DnaJ
• Protein Family: DnaJ
• UniProt Gene Name: dnaJ
• UniProt Synonym Gene Names: groP

NCBI Description

DnaK and DnaJ both bind to the same target peptide, forming a ternary complex. [More information is available at EcoGene: EG10240]. The DnaK system of Escherichia coli is a homolog of the eukaryotic Hsp70 chaperone system. [More information is available at EcoCyc: EG10240].

Uniprot Description

Interacts with DnaK and GrpE to disassemble a protein complex at the origins of replication of phage lambda and several plasmids. Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.