SDS-Page
Dnak recombinant protein
Dnak, 385-546aa, E Coli, E Coli
Gene Names
dnaK; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Dnak; 385-546aa; E Coli; Chaperone protein dnaK; HSP70; groP; grpF; seg; Heat shock protein 70; Chaperone Hsp70; Co chaperone with DnaJ; dnaK; Heat shock 70 kDa protein; ; Dnak recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 25 mM Tris-HCl, pH7.5, 2 mM beta-mercaptoethanol, 1 mM EDTA.
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST
Sequence Length
638
Applicable Applications for Dnak recombinant protein
SDS-PAGE
Antigen Species
E Coli
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for Dnak recombinant protein
DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus
Product Categories/Family for Dnak recombinant protein
References
Bardwell & Craig (1984) Proc. Natl. Acad. Sci. 81, 848-852; Zhu et al., (1996) Science 272, 1606-1614.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.7kDa (163aa)
NCBI Official Full Name
chaperone Hsp70, with co-chaperone DnaJ
NCBI Official Symbol
dnaK
NCBI Official Synonym Symbols
ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg
NCBI Protein Information
chaperone Hsp70, with co-chaperone DnaJ
UniProt Protein Name
Chaperone protein DnaK
Protein Family
UniProt Gene Name
dnaK
UniProt Synonym Gene Names
groP; grpF; seg
NCBI Description
ATP-regulated binding and release of polypeptide substrates. [More information is available at EcoGene: EG10241]. Hsc56 exhibits specificity toward Hsc62, as Hsc56 does not activate DnaK or Hsc66 ATPase activity . [More information is available at EcoCyc: EG10241].
Uniprot Description
Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
Research Articles on Dnak
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Product Notes
The Dnak dnak (Catalog #AAA203026) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dnak can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Dnak dnak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HST. It is sometimes possible for the material contained within the vial of "Dnak, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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