Histidine Decarboxylase Recombinant Protein | HDC recombinant protein

Recombinant Histidine Decarboxylase (HDC)

Cat. Num. AAA2030209

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Histidine Decarboxylase; Recombinant Histidine Decarboxylase (HDC); HDC recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; YLRHANSGAATDFMHWQIPL SRRFRSIKLW FVIRSFGVKN LQAHVRHGTE MAKYFESLVRSDPSFEIPAK RHLGLVVFRL KGPNCLTESV LKEIAKAGQL FLIPATIQDKLIIRFTVTSQ FTTKEDILRD WHLIQEAANL VLSQHCTSQP SPRAKNVIPPPPGTRGLSLE SVSEGGDDPA QARKIIKQPG ASLARREGGS DLETMPDPFDDCFSEEAPNT TKHKLSSFLF SYLSVQNRRK TTRSLSCNSV PMSAQKS
• Sequence Length: 656

• Applicable Applications for HDC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.5kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Tyr341~Ser597
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 158186734
• NCBI GeneID: 24443
• NCBI Accession #: NP_058712.2
• NCBI GenBank Nucleotide #: NM_017016.2
• Molecular Weight: 73,636 Da
• NCBI Official Full Name: histidine decarboxylase
• NCBI Official Synonym Full Names: histidine decarboxylase
• NCBI Official Symbol: Hdc
• NCBI Protein Information: histidine decarboxylase
• UniProt Protein Name: Histidine decarboxylase
• Protein Family: Headcase protein
• UniProt Gene Name: Hdc
• UniProt Synonym Gene Names: HDC
• UniProt Entry Name: DCHS_RAT

NCBI Description

involved in the formation of histamine from its precursor histidine [RGD, Feb 2006]

Uniprot Description

HDC: Catalyzes the biosynthesis of histamine from histidine (By similarity)

Protein type: Amino Acid Metabolism - histidine; EC 4.1.1.22; Lyase

Cellular Component: cell soma; dendrite; cytosol

Molecular Function: amino acid binding; protein homodimerization activity; pyridoxal phosphate binding; histidine decarboxylase activity

Biological Process: catecholamine biosynthetic process; histamine biosynthetic process; histamine metabolic process; histidine metabolic process; histidine catabolic process