Feline Sarcoma Oncogene Recombinant Protein | FES recombinant protein

Recombinant Feline Sarcoma Oncogene (FES)

Cat. Num. AAA2030061

• Gene Names: FES; FPS
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Feline Sarcoma Oncogene; Recombinant Feline Sarcoma Oncogene (FES); FES recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.9 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- D NLYRLEGDGF PSIPLLITHL LSSQQPLTKK SGVVLFRAVP KDKWVLKHED LVLGEQIGRG NFGEVFSGRL RADNTPVAVK SCRETLPPDL KAKFLQEARI LKQYNHPNIV RLIGVCTQKQ PIYIVMELVQ GGDFLTFLRT EGARLRVKTL LQMVGDAAAG MEYLESKCCI HRDLAARNCL VTEKNVLKIS DFGMSREEADGIYAASAGLR QVPVKWTAPE ALNYGRYSSE SDVWSFGILL WETFSLGASP YPNLTNQQTR
• Sequence Length: 822

• Applicable Applications for FES recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 33.0kDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Asp510~Arg770
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 23271525
• NCBI GeneID: 2242
• Molecular Weight: 85,466 Da
• NCBI Official Full Name: Feline sarcoma oncogene
• NCBI Official Synonym Full Names: FES proto-oncogene, tyrosine kinase
• NCBI Official Symbol: FES
• NCBI Official Synonym Symbols: FPS
• NCBI Protein Information: tyrosine-protein kinase Fes/Fps
• UniProt Protein Name: Tyrosine-protein kinase Fes/Fps
• Protein Family: (-)-endo-fenchol synthase
• UniProt Gene Name: FES
• UniProt Synonym Gene Names: FPS
• UniProt Entry Name: FES_HUMAN

NCBI Description

This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

Fes: a non-receptor tyrosine kinase of the Fer family. Appears to be involved in normal hematopoiesis as well as the development of acute promyelocytic leukemia. Contains one SH2 and one FCH domain. Four LOF point mutations seen in colorectal cancer. Orthologous to v-fes from feline leukemia virus and v-fps from avian transforming virus. Mutant forms are angiogenic. Promotes survival during differentiation, and may act both to promote and inhibit tumors. May be disrupted in the t(15q+;17q-) found in acute promyelocytic leukemia, but the breakpoint does not occur within the gene.

Protein type: EC 2.7.10.2; Tumor suppressor; Protein kinase, tyrosine (non-receptor); Kinase, protein; Oncoprotein; Protein kinase, TK; TK group; Fer family

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: microtubule cytoskeleton; Golgi apparatus; extrinsic to internal side of plasma membrane; focal adhesion; cytoplasmic membrane-bound vesicle; cytoplasm; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; phosphoinositide binding; ATP binding

Biological Process: regulation of mast cell degranulation; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; axon guidance; cell migration; peptidyl-tyrosine phosphorylation; positive regulation of myeloid cell differentiation; protein amino acid autophosphorylation; multicellular organismal development; positive regulation of microtubule polymerization; chemotaxis; protein amino acid phosphorylation; regulation of cell proliferation; cell proliferation; regulation of cell shape; regulation of cell differentiation; innate immune response