Bone Morphogenetic Protein 4 Recombinant Protein | BMP4 recombinant protein

Recombinant Bone Morphogenetic Protein 4 (BMP4)

Cat. Num. AAA2030034

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Bone Morphogenetic Protein 4; Recombinant Bone Morphogenetic Protein 4 (BMP4); BMP4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 9 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SHASLIPET GKKKVAEIQG HAGGRRSGQS HELLRDFEAT LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEQIH STGLEYPERP ASRANTVRSF HHEEHLENIP GTSENSAFRF LFNLSSIPEN EVISSAELRL FREQVDQGPD WERGFHRINI YEVMKPPAEV VPGHLITRLL DTRLVHHNVT RWETFDVSPA VLRWTREKQP NYGLAIEVTH LHQTRTHQGQ HVRISRSLPQ GSGNWAQLRP LLVTFGHDGR GHALTRRRRA KRSPKHHSQR ARKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN STNHAIVQTL VNSVNSSIPK ACCVPTELSA ISMLYLDEYDKVVLKNYQEM VVEGCGCR
• Sequence Length: 402

• Applicable Applications for BMP4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 48.2kDa
• Accurate Molecular Mass (KD): 48kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ser22~Arg408
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 1311542
• NCBI GeneID: 652
• Molecular Weight: 46,555 Da
• NCBI Official Full Name: bone morphogenetic protein 4
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q22-q23

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity

Biological Process: negative regulation of MAP kinase activity; renal system process; extracellular matrix organization and biogenesis; macrophage differentiation; activation of MAPKK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; lymphoid progenitor cell differentiation; telencephalon regionalization; post-embryonic development; germ cell development; regulation of protein import into nucleus; BMP signaling pathway; positive regulation of endothelial cell differentiation; positive chemotaxis; erythrocyte differentiation; chondrocyte differentiation; mesonephros development; kidney development; regulation of odontogenesis of dentine-containing teeth; endochondral ossification; negative regulation of immature T cell proliferation in the thymus; positive regulation of cardiac muscle fiber development; specification of organ position; monocyte differentiation; neuron fate commitment; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; branching morphogenesis of a tube; positive regulation of epidermal cell differentiation; negative regulation of mitosis; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; steroid hormone mediated signaling; hemopoietic progenitor cell differentiation; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of protein binding; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; cloacal septation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of cell cycle; odontogenesis; negative regulation of cell proliferation; smooth muscle development; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; regulation of smooth muscle cell differentiation; dorsoventral neural tube patterning; positive regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; smoothened signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; telencephalon development; blood vessel endothelial cell proliferation during sprouting angiogenesis; pituitary gland development; ureteric bud branching; neural tube closure; regulation of cell fate commitment; positive regulation of protein amino acid phosphorylation; positive regulation of neuron differentiation; negative regulation of myoblast differentiation; mesodermal cell fate determination; anterior/posterior axis specification

Disease: Orofacial Cleft 11; Microphthalmia, Syndromic 6