Secreted Frizzled Related Protein 1 Recombinant Protein | SFRP1 recombinant protein

Recombinant Secreted Frizzled Related Protein 1 (SFRP1)

Cat. Num. AAA2029442

• Gene Names: Sfrp1; sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Secreted Frizzled Related Protein 1; Recombinant Secreted Frizzled Related Protein 1 (SFRP1); SFRP1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; SEYDYVSFQ SDIGPYQSGR FYTKPPQCVD IPADLRLCHN VGYKKMVLPN LLEHETMAEV KQQASSWVPL LNKNCHAGTQ VFLCSLFAPV CLDRPIYPCR WLCEAVRDSC EPVMQFFGFY WPEMLKCDKF PEGDVCIAMT PPNATEASKP QGTTVCPPCD NELKSEAIIE HLCASEFALR MKIKEVKKEN GDKKIVPKKK KPLKLGPIKK KDLKKLVLYL KNGADCPCHQ LDNLSHHFLIMGRKVKSQYL LTAIHKWDKK NKEFKNFMKKMKNHECPTFQ SVFK
• Sequence Length: 314

• Applicable Applications for SFRP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 36.2kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Phe124
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 65301472
• NCBI GeneID: 20377
• NCBI Accession #: NP_038862.2
• NCBI GenBank Nucleotide #: NM_013834.3
• Molecular Weight: 35,413 Da
• NCBI Official Full Name: secreted frizzled-related protein 1
• NCBI Official Synonym Full Names: secreted frizzled-related protein 1
• NCBI Official Symbol: Sfrp1
• NCBI Official Synonym Symbols: sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik
• NCBI Protein Information: secreted frizzled-related protein 1
• UniProt Protein Name: Secreted frizzled-related protein 1
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: Sfrp1
• UniProt Synonym Gene Names: sFRP-1
• UniProt Entry Name: SFRP1_MOUSE

Uniprot Description

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; extracellular region; plasma membrane; intracellular; cytosol

Molecular Function: heparin binding; identical protein binding; Wnt-protein binding; protein binding; frizzled binding; cysteine-type endopeptidase activity; drug binding

Biological Process: neural tube development; negative regulation of osteoclast differentiation; negative regulation of Wnt receptor signaling pathway; somatic stem cell maintenance; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; proteolysis; response to organic cyclic substance; negative regulation of insulin secretion; negative regulation of BMP signaling pathway; anterior/posterior pattern formation; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of ossification; positive regulation of smoothened signaling pathway; hemopoiesis; negative regulation of osteoblast differentiation; cell differentiation; positive regulation of Wnt receptor signaling pathway; negative regulation of cell migration; female gonad development; negative regulation of epithelial cell proliferation; negative regulation of B cell differentiation; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of JNK activity; somitogenesis; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; male gonad development; digestive tract morphogenesis; positive regulation of cell growth; development of primary male sexual characteristics; negative regulation of fibroblast proliferation; neural tube closure; negative regulation of bone remodeling; positive regulation of fat cell differentiation; negative regulation of osteoblast proliferation; neural crest cell fate commitment; hemopoietic progenitor cell differentiation; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; regulation of ossification; actin cytoskeleton organization and biogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis