Integrin Beta 5 Recombinant Protein | ITGb5 recombinant protein

Recombinant Integrin Beta 5 (ITGb5)

Cat. Num. AAA2029180

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Integrin Beta 5; Recombinant Integrin Beta 5 (ITGb5); ITGb5 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.3 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-PVDL YYLMDLSLSM KDDLDNIRSL GTKLAEEMRK LTSNFRLGFG SFVDKDISPF SYTAPRYQTN PCIGYKLFPN CVPSFGFRHL LPLTDRVDSF NEEVRKQRVS RNRDAPEGGF DAVLQAAVCK EKIGWRKDAL HLLVFTTDDV PHIALDGKLG GLVQPHDGQC HLNEANEYTA SNQMDYPSLA LLGEKLAENNINLIFAVTKN HYMLYKNFTA LIPGTTVEIL DGDSKNIIQL IINAYNSI
• Sequence Length: 799

• Applicable Applications for ITGb5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.1kDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Pro137~Ile378
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 20127446
• NCBI GeneID: 3693
• NCBI Accession #: NP_002204.2
• NCBI GenBank Nucleotide #: NM_002213.4
• Molecular Weight: 88,054 Da
• NCBI Official Full Name: integrin beta-5
• NCBI Official Synonym Full Names: integrin, beta 5
• NCBI Official Symbol: ITGB5
• NCBI Protein Information: integrin beta-5
• UniProt Protein Name: Integrin beta-5
• Protein Family: Integrin
• UniProt Gene Name: ITGB5
• UniProt Entry Name: ITB5_HUMAN

Uniprot Description

ITGB5: Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. Heterodimer of an alpha and a beta subunit. Beta-5 associates with alpha-V. Interacts with MYO10. Belongs to the integrin beta chain family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 3q21.2

Cellular Component: focal adhesion; cell surface; leading edge; plasma membrane; phagocytic vesicle; receptor complex

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; antigen processing and presentation of peptide antigen via MHC class I; muscle contraction; cell-matrix adhesion; transforming growth factor beta receptor signaling pathway; stress fiber formation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I