Lectin Like Oxidized Low Density Lipoprotein Receptor 1 Recombinant Protein | LOX1 recombinant protein

Recombinant Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1)

Cat. Num. AAA2029015

• Gene Names: OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Lectin Like Oxidized Low Density Lipoprotein Receptor 1; Recombinant Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1); LOX1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.8 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TLIVQQTQLL QVSDLLKQYQ ANLTQQDHIL EGQMSAQKKA ENASQESKRE LKEQIDTLTW KLNEKSKEQE KLLQQNQNLQ EALQRAVNAS EESKWELKEQ IDILNWKLNG ISKEQKELLQ QNQNLQEALQ KAEKYSEESQ RELKEQIDTL SWKLNEKSKE QEELLQQNQN LQEALQRAAN SSGPCPQDWI WHKENCYLFH GPFNWEKSRE NCLSLDAQLL QISTTDDLNF VLQATSHSTS PFWMGLHRKNPNHPWLWENG SPLS
• Sequence Length: 273

• Applicable Applications for LOX1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 34.8kDa
• Accurate Molecular Mass (KD): 35kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Thr51~Ser314
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4468344
• NCBI GeneID: 4973
• UniProt Accession #: P78380; Q2PP00; Q7Z484; A8K7V9; B4DI48; G3V1I4
• Molecular Weight: 21,425 Da
• NCBI Official Full Name: LOX1
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: OLR1
• NCBI Official Synonym Symbols: LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: OLR1
• UniProt Synonym Gene Names: CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1
• UniProt Entry Name: OLR1_HUMAN

NCBI Description

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; receptor complex; lipid raft

Molecular Function: low-density lipoprotein receptor activity; protein binding; carbohydrate binding

Biological Process: cell death; receptor-mediated endocytosis; leukocyte adhesion; response to hydrogen peroxide; blood circulation; lipoprotein metabolic process; blood coagulation; proteolysis; inflammatory response; leukocyte migration

Disease: Myocardial Infarction, Susceptibility To