Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 2ug/mL Rabbit Anti-Rat A2BP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Ataxin 2 Binding Protein 1 Polyclonal Antibody | anti-A2BP1 antibody
Polyclonal Antibody to Ataxin 2 Binding Protein 1 (A2BP1)
Gene Names
Rbfox1; A2bp1
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Ataxin 2 Binding Protein 1; Polyclonal Antibody; Polyclonal Antibody to Ataxin 2 Binding Protein 1 (A2BP1); anti-A2BP1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against A2BP1. It has beenselected for its ability to recognize A2BP1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DP DLRQMFGQFG KILDVEIIFN ERGSKGFGFV TFENSADADR AREKLHGTVV EGRKIEVNNA TARVMTNKKA VNPYTNGWKL NPVVGAVYSP DFYAGTVLLC QANQEGSSMY SGPSSLVYTS AMPGFPYPAA TAAAAYRGAH LRGRGRTVYN TFRAAAPPPP IPAYGGVVYQ DGFYGADIYG GYAAYRYAQP TPATAAAYSD
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DP DLRQMFGQFG KILDVEIIFN ERGSKGFGFV TFENSADADR AREKLHGTVV EGRKIEVNNA TARVMTNKKA VNPYTNGWKL NPVVGAVYSP DFYAGTVLLC QANQEGSSMY SGPSSLVYTS AMPGFPYPAA TAAAAYRGAH LRGRGRTVYN TFRAAAPPPP IPAYGGVVYQ DGFYGADIYG GYAAYRYAQP TPATAAAYSD
Sequence Length
370
Applicable Applications for anti-A2BP1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
A2BP1 (Asp149~Ser399)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant A2BP1 (Asp149~Ser399) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2056521
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 2ug/mL Rabbit Anti-Rat A2BP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 2ug/mL Rabbit Anti-Rat A2BP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,556 Da
NCBI Official Full Name
RNA binding protein fox-1 homolog 1
NCBI Official Synonym Full Names
RNA binding fox-1 homolog 1
NCBI Official Symbol
Rbfox1
NCBI Official Synonym Symbols
A2bp1
NCBI Protein Information
RNA binding protein fox-1 homolog 1
UniProt Protein Name
RNA binding protein fox-1 homolog
UniProt Gene Name
Rbfox1
NCBI Description
The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. [provided by RefSeq, Nov 2011]
Uniprot Description
RNA-binding protein that regulates alternative splicing events.
Research Articles on A2BP1
Similar Products
Product Notes
The A2BP1 rbfox1 (Catalog #AAA2028774) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Ataxin 2 Binding Protein 1 (A2BP1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ataxin 2 Binding Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the A2BP1 rbfox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DP DLRQMFGQFG KILDVEIIFN ERGSKGFGFV TFENSADADR AREKLHGTVV EGRKIEVNNA TARVMTNKKA VNPYTNGWKL NPVVGAVYSP DFYAGTVLLC QANQEGSSMY SGPSSLVYTS AMPGFPYPAA TAAAAYRGAH LRGRGRTVYN TFRAAAPPPP IPAYGGVVYQ DGFYGADIYG GYAAYRYAQP TPATAAAYSD. It is sometimes possible for the material contained within the vial of "Ataxin 2 Binding Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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