Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Lung Tissue; Lane3: Human Hela Cells; Lane4: Human 293T Cells.)
Rabbit anti-Mouse Glutamate Dehydrogenase 1 Polyclonal Antibody | anti-GDH antibody
Polyclonal Antibody to Glutamate Dehydrogenase 1 (GDH)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NFFKMV EGFFDRGASI VEDKLVEDLK TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Lung Tissue; Lane3: Human Hela Cells; Lane4: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Lung Tissue; Lane3: Human Hela Cells; Lane4: Human 293T Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GDH, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GDH, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Spleen Tissue)
NCBI and Uniprot Product Information
Uniprot Description
GLUD1: May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Homohexamer. Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme. Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 1.4.1.3; Energy Metabolism - nitrogen; Mitochondrial; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Oxidoreductase
Chromosomal Location of Human Ortholog: 14 B|14 20.8 cM
Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrial matrix; mitochondrion
Molecular Function: ADP binding; ATP binding; enzyme binding; glutamate dehydrogenase [NAD(P)+] activity; glutamate dehydrogenase activity; GTP binding; NAD+ binding; nucleotide binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor; protein binding
Biological Process: amino acid metabolic process; glutamate biosynthetic process; glutamate catabolic process; glutamine metabolic process; positive regulation of insulin secretion; response to aluminum ion; tricarboxylic acid metabolic process
Research Articles on GDH
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Product Notes
The GDH glud1 (Catalog #AAA2027811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutamate Dehydrogenase 1 (GDH) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate Dehydrogenase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GDH glud1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NFFKMV EGFFDRGASI VEDKLVEDLK TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI. It is sometimes possible for the material contained within the vial of "Glutamate Dehydrogenase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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