Western Blot (WB)
(Western Blot: Sample: Recombinant HIPK2, Human.)
Rabbit anti-Human Homeodomain Interacting Protein Kinase 2 Polyclonal Antibody | anti-HIPK2 antibody
Polyclonal Antibody to Homeodomain Interacting Protein Kinase 2 (HIPK2)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant HIPK2, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HIPK2, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
HIPK2: homeodomain interacting protein kinase 2 is a CMGC kinase of the DYRK family. Interacts with TRADD. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB. Highly expressed in neuronal tissues, heart and kidney. Weakly expressed ubiquitously. Possible tumor suppressor. Activates p53 in response to UV light, causing growth arrest and apoptosis. Required for cisplatin-induced apoptosis. Downregulated in breast and thyroid carcinomas. Three alternatively spliced isoforms have been described.
Protein type: CMGC group; DYRK family; EC 2.7.11.1; HIPK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: cytoplasm; nuclear body; nucleoplasm; nucleus; PML body; RNA polymerase II transcription factor complex
Molecular Function: ATP binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; SMAD binding; transcription corepressor activity; virion binding
Biological Process: adult walking behavior; anterior/posterior pattern specification; cellular response to hypoxia; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; embryonic camera-type eye morphogenesis; embryonic retina morphogenesis in camera-type eye; erythrocyte differentiation; eye development; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of BMP signaling pathway; negative regulation of neuron apoptosis; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; PML body organization and biogenesis; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of JNK cascade; positive regulation of protein binding; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of transforming growth factor beta receptor signaling pathway; protein amino acid phosphorylation; regulation of cell cycle; retina layer formation; smoothened signaling pathway; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; virus-host interaction; voluntary musculoskeletal movement
Research Articles on HIPK2
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Product Notes
The HIPK2 hipk2 (Catalog #AAA2027424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Homeodomain Interacting Protein Kinase 2 (HIPK2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Homeodomain Interacting Protein Kinase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the HIPK2 hipk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Homeodomain Interacting Protein Kinase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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