Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Glycosylphosphatidylinositol Specific Phospholipase D1 Polyclonal Antibody | anti-GPLD1 antibody
Polyclonal Antibody to Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSSSP NITISCQDIY CNLGWTLLAA DVNGDSEPDL VIGSPFAPGG GKQKGIVAAF YSGPSLSDKE KLNVEAANWT VRGEEDFSWF GYSLHGVTVD NRTLLLVGSP TWKNASRLGH LLHIRDEKKS LGRVYGYFPP NGQSWFTISG DKAMGKLGTS LSSGHVLMNG TLKQVLLVGA PTYDDVSKVA FLTVTLHQGG ATRMYALTSD AQPLLLSTFS GDRRFSRFGG VLHLSDLDDD GLDEIIMAAP LRIADVTSGL IGGEDGRVYV YNGKETTLGD MTGKCKSWIT PCPEEKAQYV LISPEASSRF GSSLITVRSK
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue))
NCBI and Uniprot Product Information
NCBI Description
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]
Uniprot Description
GPLD1: This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Belongs to the GPLD1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.4.50; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Phospholipase; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6p22.3
Cellular Component: cytoplasm; extracellular exosome; extracellular region; extracellular space; intracellular; intracellular membrane-bound organelle; lysosomal membrane; proteinaceous extracellular matrix
Molecular Function: glycosylphosphatidylinositol phospholipase D activity; phospholipase D activity; sodium channel regulator activity
Biological Process: C-terminal protein lipidation; cell migration during sprouting angiogenesis; cellular response to calcium ion; cellular response to cholesterol; cellular response to insulin stimulus; cellular response to pH; cellular response to triglyceride; chondrocyte differentiation; complement receptor mediated signaling pathway; GPI anchor release; insulin receptor signaling pathway; negative regulation of cell proliferation; negative regulation of triglyceride catabolic process; ossification; phosphatidylcholine metabolic process; positive regulation of alkaline phosphatase activity; positive regulation of apoptosis; positive regulation of cytolysis; positive regulation of endothelial cell migration; positive regulation of glucose metabolic process; positive regulation of high-density lipoprotein particle clearance; positive regulation of membrane protein ectodomain proteolysis; positive regulation of secretion; positive regulation of triglyceride biosynthetic process; regulation of cellular response to insulin stimulus; response to glucose stimulus
Research Articles on GPLD1
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Product Notes
The GPLD1 gpld1 (Catalog #AAA2027343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glycosylphosphatidylinositol Specific Phospholipase D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the GPLD1 gpld1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSSSP NITISCQDIY CNLGWTLLAA DVNGDSEPDL VIGSPFAPGG GKQKGIVAAF YSGPSLSDKE KLNVEAANWT VRGEEDFSWF GYSLHGVTVD NRTLLLVGSP TWKNASRLGH LLHIRDEKKS LGRVYGYFPP NGQSWFTISG DKAMGKLGTS LSSGHVLMNG TLKQVLLVGA PTYDDVSKVA FLTVTLHQGG ATRMYALTSD AQPLLLSTFS GDRRFSRFGG VLHLSDLDDD GLDEIIMAAP LRIADVTSGL IGGEDGRVYV YNGKETTLGD MTGKCKSWIT PCPEEKAQYV LISPEASSRF GSSLITVRSK. It is sometimes possible for the material contained within the vial of "Glycosylphosphatidylinositol Specific Phospholipase D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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