Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Centromere Protein I Polyclonal Antibody | anti-CENPI antibody
Polyclonal Antibody to Centromere Protein I (CENPI)
Gene Names
Cenpi; CENP-I; Fshprh1
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Centromere Protein I; Polyclonal Antibody; Polyclonal Antibody to Centromere Protein I (CENPI); anti-CENPI antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CENPI. It has beenselected for its ability to recognize CENPI in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-TASLFDYLSS ERSLPLEQLQ RFPQLLESIH CLELPSQMCS VLNSPLLLHY INCVKDESIL LRISYWLSQA LQEECVWYNI NNYEQEKEFI NFLDLVIRVQ CFLQEGFYSC EAFLYKSLPL WDGSSCRSQY LQLLAWIPFS SFSEVKPLLS DHLAPLFFTS SIYFKCSVLQ SLQELLQNWL LWLSTDAHVQ PTTDSPLETT LGGSMSSVSQ LIEYTGWLCV VAMRLESSST LLLHFILDFY EKVCDIYINY
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-TASLFDYLSS ERSLPLEQLQ RFPQLLESIH CLELPSQMCS VLNSPLLLHY INCVKDESIL LRISYWLSQA LQEECVWYNI NNYEQEKEFI NFLDLVIRVQ CFLQEGFYSC EAFLYKSLPL WDGSSCRSQY LQLLAWIPFS SFSEVKPLLS DHLAPLFFTS SIYFKCSVLQ SLQELLQNWL LWLSTDAHVQ PTTDSPLETT LGGSMSSVSQ LIEYTGWLCV VAMRLESSST LLLHFILDFY EKVCDIYINY
Sequence Length
756
Applicable Applications for anti-CENPI antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CENPI (Thr321~Pro579)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant CENPI (Thr321~Pro579) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2039953
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Brain tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,403 Da
NCBI Official Full Name
centromere protein I
NCBI Official Synonym Full Names
centromere protein I
NCBI Official Symbol
Cenpi
NCBI Official Synonym Symbols
CENP-I; Fshprh1
NCBI Protein Information
centromere protein I
UniProt Protein Name
Centromere protein I
Protein Family
UniProt Gene Name
Cenpi
UniProt Synonym Gene Names
Fshprh1; CENP-I
NCBI Description
This gene encodes a protein that may be constitutively associated with centromeres and might be involved in recruiting centromere protein A to the centromere for kinetochore assembly. A similar gene in human is involved in the response of gonadal tissues to follicle-stimulating hormone. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]
Uniprot Description
CENPI: involved in the response of gonadal tissues to follicle-stimulating hormone. Highly expressed in testis, ovary and spleen.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: X|X E3
Cellular Component: chromosome; chromosome, centromeric region; cytosol; kinetochore; nuclear body; nucleus
Biological Process: centromere complex assembly
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Product Notes
The CENPI cenpi (Catalog #AAA2027114) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Centromere Protein I (CENPI) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Centromere Protein I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CENPI cenpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-TAS LFDYLSS ERSLPLEQLQ RFPQLLESIH CLELPSQMCS VLNSPLLLHY INCVKDESIL LRISYWLSQA LQEECVWYNI NNYEQEKEFI NFLDLVIRVQ CFLQEGFYSC EAFLYKSLPL WDGSSCRSQY LQLLAWIPFS SFSEVKPLLS DHLAPLFFTS SIYFKCSVLQ SLQELLQNWL LWLSTDAHVQ PTTDSPLETT LGGSMSSVSQ LIEYTGWLCV VAMRLESSST LLLHFILDFY EKVCDIYINY. It is sometimes possible for the material contained within the vial of "Centromere Protein I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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