Cavia anti-Rabbit Major Histocompatibility Complex Class II DR Alpha Polyclonal Antibody | anti-MHCDRa antibody

Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

Cat. Num. AAA2026416

• Reactivity: Rabbit
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific Affinity Chromatography
• Synonyms: Major Histocompatibility Complex Class II DR Alpha; Polyclonal Antibody; Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa); anti-MHCDRa antibody

• Host: Cavia
• Reactivity: Rabbit
• Clonality: Polyclonal
• Specificity: The antibody is a Guinea polyclonal antibody raised against MHCDRa. It has been selected for its ability to recognize MHCDRa in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific Affinity Chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
• Concentration: 500ug/mL (varies by lot)

• Applicable Applications for anti-MHCDRa antibody: WB; IHC; ICC; IP
• Application Notes: Western blotting: 0.5-5µg/mL; Immunohistochemistry: 5-20µg/mL; Immunocytochemistry: 5-20µg/mL; Optimal working dilutions must be determined by end user
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Immunogen: Recombinant MHCDRa (Ala26~Cys221) expressed in E.coli
• Cross Reactivity: Rabbit
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 4°C for frequent use.; Aliquot and store at -20°C for 12 months.; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°Cfor 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Lane1: Rabbit Spleen Tissue; Lane2: Rabbit Lymph Tissue; Lane3: Rabbit Thymus Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant MHCDRa, Rabbit.)

NCBI and Uniprot Product Information

• NCBI GI #: 283837808
• NCBI GeneID: 100328929
• NCBI Accession #: NP_001164589.1
• NCBI GenBank Nucleotide #: NM_001171118.1
• UniProt Accession #: Q30847; G1SWJ6
• Molecular Weight: 28,622 Da
• NCBI Official Full Name: major histocompatibility complex, class II, DR alpha
• NCBI Official Symbol: RLA-DR-ALPHA
• NCBI Protein Information: major histocompatibility complex, class II, DR alpha
• UniProt Protein Name: Integral membrane protein
• UniProt Gene Name: RLA-DR-alpha

NCBI Description

HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DRB5: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; extracellular space; membrane; late endosome membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; MHC class II protein complex; external side of plasma membrane

Molecular Function: peptide antigen binding

Biological Process: humoral immune response mediated by circulating immunoglobulin; T-helper 1 type immune response; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; detection of bacterium; regulation of interleukin-4 production; cytokine and chemokine mediated signaling pathway; negative regulation of interferon-gamma production; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; immunoglobulin production during immune response; T cell receptor signaling pathway; protein tetramerization