Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Human HepG2 Cells; Lane4: Human U2OS Cells; Lane5: Human A549 Cells.)
Rabbit anti-Human Protein Kinase D1 Polyclonal Antibody | anti-PKD1 antibody
Polyclonal Antibody to Protein Kinase D1 (PKD1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TPERVFVVME KLHGDMLEMI LSSEKGRLPE HITKFLITQI LVALRHLHFK NIVHCDLKPE NVLLASADPF PQVKLCDFGF ARIIGEKSFR RSVVGTPAYL APEVLRNKGY NRSLDMWSVG VIIYVSLSGT FPFNEDEDIH DQIQNAAFMY PPNPWKEISH EAIDLINNLL QVKMRKRYSV DKTLSHPWLQ DYQTWLDLRE LECKIGERYI THESDDLRWE KYAGEQ
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Human HepG2 Cells; Lane4: Human U2OS Cells; Lane5: Human A549 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Human HepG2 Cells; Lane4: Human U2OS Cells; Lane5: Human A549 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PKD1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PKD1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]
Uniprot Description
PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A
Protein type: Autophagy; CAMK group; EC 2.7.11.13; Kinase, protein; Motility/polarity/chemotaxis; PKD family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: autophagosome membrane; cytosol; Golgi apparatus; integral component of plasma membrane; plasma membrane; trans-Golgi network
Molecular Function: ATP binding; identical protein binding; kinase activity; metal ion binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity
Biological Process: angiogenesis; apoptosis; cell proliferation; cellular response to amino acid starvation; cellular response to hydroperoxide; cellular response to oxidative stress; Golgi organization and biogenesis; Golgi vesicle transport; inflammatory response; innate immune response; integrin-mediated signaling pathway; negative regulation of endocytosis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of blood vessel endothelial cell migration; positive regulation of CREB transcription factor activity; positive regulation of endothelial cell chemotaxis; positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway; positive regulation of endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of neuron projection development; positive regulation of NF-kappaB transcription factor activity; positive regulation of osteoblast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; protein autophosphorylation; protein kinase D signaling; Ras protein signal transduction; regulation of integrin-mediated signaling pathway; regulation of keratinocyte proliferation; regulation of protein stability; signal transduction; sphingolipid biosynthetic process; vascular endothelial growth factor receptor signaling pathway
Disease: Congenital Heart Defects And Ectodermal Dysplasia
Research Articles on PKD1
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Product Notes
The PKD1 prkd1 (Catalog #AAA2026250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Protein Kinase D1 (PKD1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Protein Kinase D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PKD1 prkd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TPERVFVVME KLHGDMLEMI LSSEKGRLPE HITKFLITQI LVALRHLHFK NIVHCDLKPE NVLLASADPF PQVKLCDFGF ARIIGEKSFR RSVVGTPAYL APEVLRNKGY NRSLDMWSVG VIIYVSLSGT FPFNEDEDIH DQIQNAAFMY PPNPWKEISH EAIDLINNLL QVKMRKRYSV DKTLSHPWLQ DYQTWLDLRE LECKIGERYI THESDDLRWE KYAGEQ. It is sometimes possible for the material contained within the vial of "Protein Kinase D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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