Human Adrenomedullin 2 (ADM2) ELISA Kit | ADM2 elisa kit

Human Adrenomedullin 2 (ADM2) ELISA Kit

Cat. Num. AAA2025041

• Gene Names: ADM2; AM2; dJ579N16.4
• Reactivity: Human
• Synonyms: Adrenomedullin 2 (ADM2); Human Adrenomedullin 2 (ADM2) ELISA Kit; AM2; IMDL; IMDS; Intermedin; ADM2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenomedullin 2 (ADM2).; No significant cross-reactivity or interference between Adrenomedullin 2 (ADM2) and analogues was observed.
• Sequence Length: 148

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 246.9-20000pg/mL
• Sensitivity: < 89.4pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin 2 (ADM2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin 2 (ADM2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADM2 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenomedullin 2 (ADM2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenomedullin 2 (ADM2) and unlabeled Adrenomedullin 2 (ADM2) (Standards or samples) with the pre-coated antibody specific to Adrenomedullin 2 (ADM2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenomedullin 2 (ADM2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenomedullin 2 (ADM2) in the sample.

Product Categories/Family for ADM2 elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 77362698
• NCBI GeneID: 79924
• Molecular Weight: 15,865 Da
• NCBI Official Full Name: adrenomedullin 2
• NCBI Official Synonym Full Names: adrenomedullin 2
• NCBI Official Symbol: ADM2
• NCBI Official Synonym Symbols: AM2; dJ579N16.4
• NCBI Protein Information: ADM2
• UniProt Protein Name: ADM2
• Protein Family: ADM
• UniProt Gene Name: ADM2
• UniProt Synonym Gene Names: AM2; AM2; IMDL; IMDS
• UniProt Entry Name: ADM2_HUMAN

NCBI Description

This gene encodes a member of the calcitonin gene-related peptide (CGRP)/calcitonin family of hormones that play a role in the regulation of cardiovascular homeostasis, prolactin release, anti-diuresis, anti-natriuresis, and regulation of food and water intake. The encoded protein is proteolytically processed to generate one or more biologically active peptides. [provided by RefSeq, Jul 2015]

Uniprot Description

ADM2: IMDL and IMDS may play a role as physiological regulators of gastrointestinal, cardiovascular bioactivities mediated by the CALCRL/RAMPs receptor complexes. Activates the cAMP-dependent pathway. Belongs to the adrenomedullin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: extracellular region

Molecular Function: hormone activity; protein complex binding

Biological Process: positive regulation of angiogenesis; digestion; feeding behavior; angiogenesis; negative regulation of blood pressure; protein amino acid phosphorylation; G-protein signaling, adenylate cyclase activating pathway